Rats born to Brucella abortus infected mothers become latent carriers of Brucella

Authors

  • Md. Ariful Islam Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Mst. Minara Khatun Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Byeong-Kirl Baek College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

DOI:

https://doi.org/10.3855/jidc.1997

Keywords:

rats, Brucella abortus biotype 1, latent carrier

Abstract

Introduction: Rats are known to be infected with Brucella. Vertical transmission of brucellosis was recorded in rats. The study was performed to judge whether rats born from Brucella abortus infected mothers can act as latent carriers of Brucella infection.

Methodology: Female Sprague Dawley (SD) rats were experimentally infected with B. abortus biotype 1 and subsequently bred 10 days post infection (PI). Serum samples of rats (n = 48) born from infected dams were tested using the Rose Bengal plate test (RBPT), tube agglutination test (TAT), and enzyme-linked immunosorbent assay (ELISA) at one, two and three months of age. Tissue samples were plated onto Brucella agar and blood agar media and incubated at 37°C with 5% CO2 for five to seven days for isolation of bacteria..

Results: B. abortus was isolated from 18 out of 48 rats born to infected dams, and the isolates were confirmed as B. abortus by AMOS (B. abortus, melitensis, ovis and suis) PCR assay with the production of a 498 bp PCR amplicon. Serum samples of rats (n = 48) born from infected dams were tested negative using the RBPT, TAT and ELISA at all time points.

Conclusion: We conclude from the study that rats born to infected dams may become latent carriers of Brucella infection potentially providing a reservoir for future transmission.

Author Biographies

Md. Ariful Islam, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh

Associate Professor in the Department of Microbiology & Hygiene. Major field of research : Brucellosis

 

Mst. Minara Khatun, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh

Associate Professor in the Department of Microbiology & Hygiene. Major field of research : Brucellosis and Food poisoning bacteria

Byeong-Kirl Baek, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

Professor in Veterinary Public Health at College of Veterinary Medicine, Chonbuk National University and Director, Korean Zoonoses Research Institute

Downloads

Published

2011-11-30

How to Cite

1.
Islam MA, Khatun MM, Baek B-K (2011) Rats born to Brucella abortus infected mothers become latent carriers of Brucella. J Infect Dev Ctries 6:256–261. doi: 10.3855/jidc.1997

Issue

Vol. 6 No. 03: March 2012

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC