Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait

Authors

  • Rana Al-Awadhi Faculty of Allied Health Sciences, Kuwait University, Kuwait
  • Wassim Chehadeh Faculty of Medicine, Kuwait University, Kuwait
  • Waleed Al-Jassar Faculty of Medicine, Kuwait University, Kuwait
  • Jehad Al-Harmi Faculty of Medicine, Kuwait University, Kuwait
  • Eyad Al-Saleh Faculty of Medicine, Kuwait University, Kuwait
  • Kusum Kapila Faculty of Medicine, Kuwait University, Kuwait

DOI:

https://doi.org/10.3855/jidc.2748

Keywords:

HPV, real-time PCR quantitative assay, viral load, women with normal and abnormal cervical cytology

Abstract

Introduction: Human papillomaviruses (HPV) are the most commonly known sexually transmitted agents. Almost all cases of cervical cancer are caused by persistent infection. This study was conducted to ascertain whether there is a difference in HPV load in cervical samples with normal and abnormal cervical cytology reports in Kuwait.

Methodology: HPV-positive abnormal ThinPrep samples (n = 206) and normal ThinPrep samples (n = 120) were taken from women attending gynecology clinics. Real-time PCR was used to measure the viral load for all HPV genotypes.

Results: The median normalized viral load in samples with normal and abnormal cytology reports was 0.86 × 10-7 and 4.66 × 10-7, respectively (p = 0.001). Median normalized viral load of high-risk (HR), intermediate-risk (IR) and low-risk (LR) HPV was 4.04 × 10-7, 0.71 × 10-7 and 2.38 × 10-7, respectively, (p = 0.002).

Conclusions: The findings suggest that, in the absence of a proper screening programme in Kuwait, quantification of HPV viral load could be considered as a surrogate virology test to identify women with abnormal cytology. Further population-based prospective studies are needed to include more women with high-grade and invasive carcinoma reports.

Author Biographies

Rana Al-Awadhi, Faculty of Allied Health Sciences, Kuwait University, Kuwait

Department of Medical Laboratory Sciences, Assisstant Professor

Wassim Chehadeh, Faculty of Medicine, Kuwait University, Kuwait

Department of Microbiology (Virology Unit), Associate Professor

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Published

2013-02-15

How to Cite

1.
Al-Awadhi R, Chehadeh W, Al-Jassar W, Al-Harmi J, Al-Saleh E, Kapila K (2013) Viral load of human papillomavirus in women with normal and abnormal cervical cytology in Kuwait. J Infect Dev Ctries 7:130–136. doi: 10.3855/jidc.2748

Issue

Vol. 7 No. 02: February 2013

Section

Original Articles

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