Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals

  • David Olusoga Ogbolu Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria
  • Oluwole Adebayo Daini Olabisi Onabanjo University, Remo Campus, Ago-Iwoye, Nigeria
  • Afolabi Ogunledun University, Remo Campus, Ago-Iwoye, Nigeria
  • Oyebode Armstrong Terry Alli Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria
  • Mark Alexander Webber University of Birmingham, Birmingham, United Kingdom
Keywords: ESBL, plasmid, resistant genes, bacteria, Nigeria

Abstract

Introduction: Production of beta-lactamases is the predominant cause of resistance to beta-lactam antibiotics in Gram-negative bacteria. We investigated the diversity of plasmid-borne beta-lactamase genes and replicon type of the plasmids carrying the respective genes in Gram-negative bacteria recovered from clinical infection in Nigerian hospitals.

Methodology: A total of 134 Gram-negative bacteria of 13 species were analyzed for antimicrobial susceptibility, phenotypic and genotypic detection of various beta-lactamases, and plasmid analysis, including replicon typing.

Results: Of the 134 isolates, 111 (82.8%) contained beta-lactamases, while 28 (20.9%) carried extended-spectrum beta-lactamases. PCR and sequencing identified TEM-1 in 109 isolates (81.3%), SHV-1 in 33 isolates (24.6%), OXA-1 in 15 isolates (11.2%) and CTX-M enzymes (24 CTX-M-15 and 1 CTX-M-3) in 25 isolates (18.7%). Multiplex PCR showed that 6 isolates carried plasmidic AmpCs (ACT-1, DHA-1 and CMY-2); these enzymes were detected only in isolates possessing CTX-M beta-lactamases. Of 13 (76.9%) representative plasmids investigated in detail, 9 (69.2%) were self-transferable when selected by a beta-lactam and the plasmids once transferred coded for beta-lactam resistance. Replicon typing indicated IncF as the common vector encoding for beta-lactamases.

Conclusions: The study showed a diversity of beta-lactamase genes disseminated by conjugative IncF plasmids in Gram-negative bacteria; TEM-1, SHV-1, OXA-1, CTX-M-15, CTX-M-3and plasmidic AmpC enzymes are in common circulation in Nigeria.

Author Biographies

David Olusoga Ogbolu, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria
DEPARTMENT OF BIOMEDICAL SCIENCES, LECTURER I
Oluwole Adebayo Daini, Olabisi Onabanjo University, Remo Campus, Ago-Iwoye, Nigeria
Department of Biochemistry, Reader
Afolabi Ogunledun, University, Remo Campus, Ago-Iwoye, Nigeria
Department of Medical Microbiology, Professor
Oyebode Armstrong Terry Alli, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria
DEPARTMENT OF BIOMEDICAL SCIENCES, SENIOR LECTURER
Mark Alexander Webber, University of Birmingham, Birmingham, United Kingdom
Antimicrobial Research Group, Immunity and infection, Lecturer
Published
2013-05-13
How to Cite
1.
Ogbolu DO, Daini OA, Ogunledun A, Terry Alli OA, Webber MA (2013) Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals. J Infect Dev Ctries 7:382-390. doi: 10.3855/jidc.2613
Section
Original Articles