Increasing incidence of Gram-negative organisms in bacterial agents isolated from diabetic foot ulcers

  • Vedat Turhan Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Mesut Mutluoglu Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Ali Acar Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Mustafa Hatipoglu Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Yalcın Onem Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Gunalp Uzun Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Hakan Ay Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Oral Oncul Gata Haydarpasa Training Hospital, Istanbul, Turkey
  • Levent Gorenek Gata Haydarpasa Training Hospital, Istanbul, Turkey
Keywords: diabetic foot infection, bacterial pathogens, culture, Gram-positive bacteria, Gram-negative bacteria

Abstract

Introduction: In the present study, we sought to identify the bacterial organisms associated with diabetic foot infections (DFIs) and their antibiotic sensitivity profiles.

Methodology: We retrospectively reviewed the records of wound cultures collected from diabetic patients with foot infections between May 2005 and July 2010.

Results: We identified a total of 298 culture specimens (165 [55%] wound swab, 108 [36%] tissue samples, and 25 [9%] bone samples) from 107 patients (74 [69%] males and 33 [31%] females, mean age 62 ± 13 yr) with a DFI. Among all cultures 83.5% (223/267) were monomicrobial and 16.4% (44/267) were polymicrobial. Gram-negative bacterial isolates (n = 191; 61.3%) significantly outnumbered Gram-positive isolates (n = 121; 38.7%). The most frequently isolated bacteria were Pseudomonas species (29.8%), Staphylococcus aureus (16.7%), Enterococcus species (11.5%), Escherichia coli (7.1%), and Enterobacter species (7.1%), respectively. While 13.2% of the Gram-negative isolates were inducible beta-lactamase positive, 44.2% of Staphylococcus aureus isolates were methicillin resistant.

Conclusions: Our results support the recent view that Gram-negative organisms, depending on the geographical location, may predominate in DFIs.

Author Biographies

Vedat Turhan, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Associated Professor inDept of "Infectious Diseases and Clinical Microbiology"
Mesut Mutluoglu, Gata Haydarpasa Training Hospital, Istanbul, Turkey
MD inDept of "Undersea and Hyperbaric medicine"
Ali Acar, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Associated Professor inDept of "Infectious Diseases and Clinical Microbiology"
Mustafa Hatipoglu, Gata Haydarpasa Training Hospital, Istanbul, Turkey
MD inDept of "Infectious Diseases and Clinical Microbiology"
Yalcın Onem, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Associated Professor inDept of "Internal Medicine"
Gunalp Uzun, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Associated Professor inDept of "Undersea and Hyperbaric medicine"
Hakan Ay, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Associated Professor inDept of "Undersea and Hyperbaric medicine"
Oral Oncul, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Professor inDept of "Infectious Diseases and Clinical Microbiology"
Levent Gorenek, Gata Haydarpasa Training Hospital, Istanbul, Turkey
Professor inDept of "Infectious Diseases and Clinical Microbiology"
Published
2013-10-15
How to Cite
1.
Turhan V, Mutluoglu M, Acar A, Hatipoglu M, Onem Y, Uzun G, Ay H, Oncul O, Gorenek L (2013) Increasing incidence of Gram-negative organisms in bacterial agents isolated from diabetic foot ulcers. J Infect Dev Ctries 7:707-712. doi: 10.3855/jidc.2967
Section
Original Articles