Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Ziad Daoud; Elie Salem Sokhn; Eid Azar; Khalil Masri; Shira Doron

doi:10.3855/jidc.3164

Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Authors

Ziad Daoud University of Balamand, Tripoli, Lebanon
Elie Salem Sokhn University of Surrey, Guildford, United Kingdom
Eid Azar University of Balamand, Tripoli, Lebanon
Khalil Masri Centre Hospitalier du Nord, Zgharta, Lebanon
Shira Doron Tufts Medical Center, Boston, MA,United States

DOI:

https://doi.org/10.3855/jidc.3164

Keywords:

mutant prevention concentration, ESBL, ciprofloxacin, Escherichia coli, Klebsiella pneumoniae

Abstract

Introduction: The aim of this work was to study the ability of ciprofloxacin to restrict the development of resistant mutants of Escherichia coli and Klebsiella pneumoniae through determination of the Mutant Prevention Concentration (MPC).

Methodology: We studied 140 strains of E. coli and 86 strains of K. pneumoniae with different profiles of sensitivity to fluoroquinolones and extended spectrum beta lactamase (ESBL) production. The MPCs were determined using an inoculum of 10¹⁰ CFU/ml in Mueller-Hinton agar plates with serial concentrations of ciprofloxacin.

Results: Ciprofloxacin-susceptible ESBL-producing strains showed a higher MPC for ciprofloxacin (P <0.001) than ciprofloxacin-susceptible non ESBL-producing strains, while ciprofloxacin-resistant ESBL-producing and non ESBL-producing strains did not significantly differ. The presence of qnr variants was associated with elevated MPCs. This was observed for both tested organisms.

Conclusions: Our study helps to explain the frequent finding of resistance to fluoroquinolones in ESBL-producing strains. Consequently, the use of concentrations of ciprofloxacin higher than the MIC in order to prevent the recovery and growth of resistant mutants is recommended.

Author Biography

Ziad Daoud, University of Balamand, Tripoli, Lebanon

Professor of Clinical Microbiology

Department of Biomedical Sciences

Microbiology

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Published

2014-02-13

How to Cite

Daoud Z, Sokhn ES, Azar E, Masri K, Doron S (2014) Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae. J Infect Dev Ctries 8:154–159. doi: 10.3855/jidc.3164

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Issue

Vol. 8 No. 02: February 2014

Section

Original Articles

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