Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil

  • Olivia Cometti Favalessa Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
  • Daphine Ariadne Jesus de Paula Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
  • Valeria Dutra Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
  • Luciano Nakazato Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
  • Tomoko Tadano Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
  • Marcia dos Santos Lazera IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
  • Bodo Wanke IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
  • Luciana Trilles IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
  • Maria Walderez Szeszs IAL - Instituto Adolfo Lutz – Seção de Micologia São Paulo, SP, Brazil
  • Dayane Silva IAL - Instituto Adolfo Lutz – Seção de Micologia São Paulo, SP, Brazil
  • Rosane Christine Hahn Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
Keywords: Cryptococosis, Cryptococcus complex, antifungal drugs

Abstract

Introduction: Cryptococcosis is a systemic fungal infection that affects humans and animals, mainly due to Cryptococcus neoformans and Cryptococcus gattii. Following the epidemic of acquired immunodeficiency syndrome (AIDS), fungal infections by C. neoformans have become more common among immunocompromised patients. Cryptococcus gattii has primarily been isolated as a primary pathogen in healthy hosts and occurs endemically in northern and northeastern Brazil. We to perform genotypic characterization and determine the in vitro susceptibility profile to antifungal drugs of the Cryptococcus species complex isolated from HIV-positive and HIV-negative patients attended at university hospitals in Cuiabá, MT, in the Midwestern region of Brazil.

Methodology: Micromorphological features, chemotyping with canavanine-glycine-bromothymol blue (CGB) agar and genotyping by URA5-RFLP were used to identify the species. The antifungal drugs tested were amphotericin B, fluconazole, flucytosine, itraconazole and voriconazole. Minimum inhibitory concentrations (MICs) were determined according to the CLSI methodology M27-A3.

Results: Analysis of samples yelded C. neoformans AFLP1/VNI (17/27, 63.0%) and C. gattii AFLP6/VGII (10/27, 37.0%). The MICs ranges for the antifungal drugs were: amphotericin B (0.5-1 mg/L), fluconazole (1-16 mg/L), flucytosine (1-16 mg/L), itraconazole (0.25-0.12 mg/L) and voriconazole (0.06-0.5 mg/L). Isolates of C. neoformans AFLP1/VNI were predominant in patients with HIV/AIDS, and C. gattii VGII in HIV-negative patients. The genotypes identified were susceptible to the antifungal drugs tested.

Conclusion: It is worth emphasizing that AFLP6/VGII is a predominant genotype affecting HIV-negative individuals in Cuiabá. These findings serve as a guide concerning the molecular epidemiology of C. neoformans and C. gattii in the State of Mato Grosso.

Author Biographies

Olivia Cometti Favalessa, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

Faculdade de Medicina

Laboratório de Investigação

Universidade Federal de Mato Grosso

Daphine Ariadne Jesus de Paula, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
Faculty of Veterinary Sciences
Valeria Dutra, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
Faculty of Veterinary Sciences
Luciano Nakazato, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

Faculty of Veterinary Sciences

Tomoko Tadano, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

Hospital Universitario Julio Muller

Universidade Federal de Mato Grosso

Marcia dos Santos Lazera, IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
Mycology Laboratory
Bodo Wanke, IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
Mycology Laboratory
Luciana Trilles, IPEC – Laboratório de Micologia - FIOCRUZ, Rio de Janeiro, RJ, Brazil
Mycology Laboratory
Maria Walderez Szeszs, IAL - Instituto Adolfo Lutz – Seção de Micologia São Paulo, SP, Brazil

Mycology section

Dayane Silva, IAL - Instituto Adolfo Lutz – Seção de Micologia São Paulo, SP, Brazil

Mycology section

Rosane Christine Hahn, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

Faculdade de Medicina

Laboratório de Investigação

Universidade Federal de Mato Grosso

Published
2014-08-13
How to Cite
1.
Favalessa OC, de Paula DAJ, Dutra V, Nakazato L, Tadano T, Lazera M dos S, Wanke B, Trilles L, Walderez Szeszs M, Silva D, Hahn RC (2014) Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil. J Infect Dev Ctries 8:1037-1043. doi: 10.3855/jidc.4446
Section
Original Articles