Impact of Aeromonas and diarrheagenic Escherichia coli screening in patients with diarrhea in Paraná, southern Brazil

  • Flávia E.A. Assis Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Suélen Wolf Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Monica Surek Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Fabiana De Toni Laboratório Municipal de Curitiba, Curitiba, PR, Brazil
  • Emanuel M Souza Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Fábio O Pedrosa Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Sônia M. S. S. Farah Laboratório Central do Estado do Paraná, Curitiba, PR, Brazil
  • Geraldo Picheth Universidade Federal do Paraná, Curitiba, PR, Brazil
  • Cyntia M.T. Fadel-Picheth Universidade Federal do Paraná, Curitiba, PR, Brazil
Keywords: diarrhea, bacterial enteropathogens, prevalence, laboratory diagnosis, gastroenteritis agents

Abstract

Introduction: A wide diversity of bacterial agents may cause diarrhea, presenting challenges to clinical laboratories to define a diagnosis. Considering that most stool cultures are negative, we screened stool samples from patients with diarrhea for the presence of 14 bacterial enteropathogens, aiming to establish which of them should be included in routine stool analysis.

Methodology: Stool samples from 400 patients with diarrhea were analyzed for the presence of Salmonella, Shigella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Vibrio, Yersinia enterocolitica, and diarrheagenic Escherichia coli using conventional microbiological methods and PCR. Two distinct samples were studied; one included predominantly patients involved in outbreaks, and the other patients of low socioeconomic status presenting sporadic cases of diarrhea.

Results: In total, 86 cultures (21.5%) were positive. Mixed infections were found in five patients, leading to recovery of 91 strains of enteropathogenic bacteria: Salmonella Enteritidis (9.2%), Aeromonas (7.2%), diarrheagenic E. coli (5.2%), and C. jejuni (1%). However, Salmonella predominated, with 11.5% frequency in diarrhea outbreaks, while Aeromonas predominated among patients of low socioeconomic status, with 14.6% frequency.

Conclusion: Aeromonas and diarrheagenic E. coli, which are not routinely screened for, deserve to be included in laboratory screening panels.

Author Biographies

Flávia E.A. Assis, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Análises Clínicas
Suélen Wolf, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Análises Clínicas
Monica Surek, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Análises Clínicas
Fabiana De Toni, Laboratório Municipal de Curitiba, Curitiba, PR, Brazil
Microbiologia
Emanuel M Souza, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Bioquímica e Biologia Molecular
Fábio O Pedrosa, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Bioquímica e Biologia Molecular
Sônia M. S. S. Farah, Laboratório Central do Estado do Paraná, Curitiba, PR, Brazil
Setor de Bacteriologia
Geraldo Picheth, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Análises Clínicas
Cyntia M.T. Fadel-Picheth, Universidade Federal do Paraná, Curitiba, PR, Brazil
Departamento de Análises Clínicas
Published
2014-12-15
How to Cite
1.
Assis FE, Wolf S, Surek M, De Toni F, Souza EM, Pedrosa FO, Farah SMSS, Picheth G, Fadel-Picheth CM (2014) Impact of Aeromonas and diarrheagenic Escherichia coli screening in patients with diarrhea in Paraná, southern Brazil. J Infect Dev Ctries 8:1609-1614. doi: 10.3855/jidc.4434
Section
Brief Original Articles