Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria

  • Casmir Ifeanyichukwu Cajetan Ifeanyi Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
  • Nkiruka Florence Ikeneche College of Health Science, University of Abuja, Abuja, Nigeria 3 World Health Organization Office, Abuja, Nigeria
  • Bassey Enya Bassey Laboratoire de Controle des Eaux et Denrées Alimentaires, Institut Pasteur de Tunis, Tunisia
  • Nazek Al-Gallas Laboratoire de Controle des Eaux et Denre´es Alimentaires, Institut Pasteur de Tunis, Tunisia
  • Ridha Ben Aissa Laboratoire de Controle des Eaux et Denrées Alimentaires, Institut Pasteur de Tunis, Tunisia
  • Abdellatif Boudabous Laboratoire de Microbiologie, Faculté des Sciences deTunis, Tunis, Tunisia
Keywords: diarrheagenic, virulence genes, pathotype

Abstract

Introduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown.

Methodology: Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied.E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories.

Results: Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age.

Conclusions: This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.

Author Biography

Casmir Ifeanyichukwu Cajetan Ifeanyi, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
Research Scientist Department of Microbiology Faculty of Veterinary Medicine University of Abuja, Nigeria
Published
2015-02-19
How to Cite
1.
Ifeanyi C, Ikeneche N, Bassey B, Al-Gallas N, Ben Aissa R, Boudabous A (2015) Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria. J Infect Dev Ctries 9:165-174. doi: https://doi.org/10.3855/jidc.5528
Section
Original Articles