Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

  • Rasha H. Bassyouni Faculty of Medicine, Fayoum University, Fayoum, Egypt
  • Sylvana Nady Gaber Faculty of Medicine, Fayoum University, Fayoum, Egypt
  • Ahmed Ashraf Wegdan Faculty of Medicine, Fayoum University, Fayoum, Egypt
Keywords: E. coli, ESBL, AmpC, HCWs

Abstract

Introduction: Commensal E. coli can be considered a reservoir of genes coding for antibiotic resistance that may be transmitted in hospitals by healthcare workers (HCWs). This study aimed to determine the fecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli among HCWs.

Methodology: Stool samples were collected from 200 HCWs. Phenotypic screening for ESBL and AmpC β-lactamases was performed using disk diffusion and minimum inhibitory concentration methods followed by the combined disks test and double synergy differential test for confirmation. Multiplex polymerase chain reaction (PCR) was used to detect blaSHV, blaTEM, blaCTX-M, and CIT groups for AmpC genes.

Results: Of 200 E. coli isolates, 100% were susceptible to imipenem, and 59 (29.5%) were resistant to one or more third-generation cephalosporins. By molecular analysis, 21% (42/200) were colonized by ESBL-producing E. coli, and 3% (6/200) were colonized by AmpC-producing E. coli. The blaSHVgene was the predominant ESBL gene, detected in 81.8% of the resistant E. coli isolates.

Conclusions: These findings highlight the increase in fecal carriage of E. coli carrying ESBL and AmpC genes among HCWs, which may be one of the causes of the spread of ESBL-producing bacteria in hospitals and requires sound infection control measures. This is the first study of the fecal carriage rate of E. coli carrying AmpC genes in HCWs.

Published
2015-03-15
How to Cite
1.
Bassyouni RH, Gaber SN, Wegdan AA (2015) Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers. J Infect Dev Ctries 9:304-308. doi: 10.3855/jidc.5633
Section
Brief Original Articles