Determination of PFOR gene expression in strains of G. intestinalis with different inhibitory concentrations of metronidazole

  • Rozalia Begaydarova Karaganda State Medical University, Karaganda, Kazakhstan
  • Yekaterina Yukhnevich Karaganda State Medical University, Karaganda, Kazakhstan
  • Dmitry Babenko Karaganda State Medical University, Karaganda, Kazakhstan
  • Sholpan Kaliyeva Karaganda State Medical University, Karaganda, Kazakhstan
  • Iliya Azizov Karaganda State Medical University, Karaganda, Kazakhstan
  • Gulmira Muldaeva Karaganda State Medical University, Karaganda, Kazakhstan
  • Baurzhan Omarkulov Karaganda State Medical University, Karaganda, Kazakhstan
Keywords: G. intestinalis, metronidazole resistance, pyruvate ferredoxin oxidoreductase (PFOR) expression

Abstract

Introduction: Giardia intestinalis is the most important and common diarrhea-causing parasitic protozoa worldwide with growing clinical relevance in public health. There are many documented cases of G. intestinalis resistance to metronidazole (MZ). Pyruvate: ferredoxin oxidoreductase (PFOR), the membrane-localized enzyme, plays a key role in the development of resistance to drugs. The aim of the present study was to evaluate the difference in the levels of PFOR gene expression between MZ-resistant and MZ-susceptible strains of G. intestinatlis.

Methodology: From 159 samples with G. intestinalis cysts, 48 strains were successfully cultivated. Using specific pair primers, PFOR gene expressions were estimated in different groups of Giardia. The polymerase chain reaction (PCR) data were analyzed with Bayesian analysis of qRT-PCR data using MCMC.qpcr package, with relative expression software tool (REST) and quantitative PCR CopyCount web source.

Results: In the group of Giardia with minimum inhibitory concentration (MIC) of 6.3 µM, the level of PFOR gene expression was downregulated and compared with controls, differed by 1.5 to 2.8 times. At the same time, there was no significant difference in PFOR gene expression between the control (susceptible) group and the group with MIC of 3.2 µM.

Conclusions: Though there is association between PFOR gene expression and metronidazole resistance of Giardia intestinalis, the level of PFOR gene expression cannot be a strong genetic marker to predict level of resistance to metronidazole based on MICs.

Author Biographies

Rozalia Begaydarova, Karaganda State Medical University, Karaganda, Kazakhstan
Department of children infection diseases, head of department, professor
Yekaterina Yukhnevich, Karaganda State Medical University, Karaganda, Kazakhstan
Department of evidence-based medicine and clinical pharmacology, MSc
Dmitry Babenko, Karaganda State Medical University, Karaganda, Kazakhstan
Scientific center and laboratories, MSc
Sholpan Kaliyeva, Karaganda State Medical University, Karaganda, Kazakhstan
Department of evidence-based medicine and clinical pharmacology, head of department, associate professor
Iliya Azizov, Karaganda State Medical University, Karaganda, Kazakhstan
Scientific center and laboratories, head of center, professor
Gulmira Muldaeva, Karaganda State Medical University, Karaganda, Kazakhstan
Department of general medicine 1, head of department, professor
Baurzhan Omarkulov, Karaganda State Medical University, Karaganda, Kazakhstan
Department of postgraduate education, head of department, associate professor
Published
2015-05-18
How to Cite
1.
Begaydarova R, Yukhnevich Y, Babenko D, Kaliyeva S, Azizov I, Muldaeva G, Omarkulov B (2015) Determination of PFOR gene expression in strains of G. intestinalis with different inhibitory concentrations of metronidazole. J Infect Dev Ctries 9:519-523. doi: 10.3855/jidc.5768
Section
Original Articles