Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients

  • Caio Ferreira de Oliveira Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, Brazil
  • Alexandre Tadachi Morey Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, Brazil
  • Jussevania Pereira Santos Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, Brazil
  • Ludmila Vilela Pereira Gomes Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, Brazil
  • Juscélio Donizete Cardoso Instituto Agronômico do Paraná, Departamento de Microbiologia do Solo, Londrina, Paraná, Brazil
  • Phileno Pinge-Filho Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  • Márcia Regina Eches Perugini Centro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  • Lucy Megumi Yamauchi Centro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  • Sueli Fumie Yamada-Ogattta Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, Brazil
Keywords: antimicrobial resistance, intermediate vancomycin resistance, MRSA, SCCmec typing, virulence factors

Abstract

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods.

Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes.

Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed.

Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections.

Published
2015-07-30
How to Cite
1.
Oliveira CF de, Morey AT, Santos JP, Gomes LVP, Cardoso JD, Pinge-Filho P, Perugini MRE, Yamauchi LM, Yamada-Ogattta SF (2015) Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients. J Infect Dev Ctries 9:743-751. doi: 10.3855/jidc.5868
Section
Original Articles