Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis

  • Ivy Bastos Ramis Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil
  • Júlia Silveira Vianna Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil
  • Priscila Cristina Bartolomeu Halicki Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil
  • Caroline Lara Universidade Federal do Rio Grande (FURG), Pelotas, Rio Grande, Rio Grande do Sul, Brasil
  • Thássia Fernanda Tadiotto Universidade Federal do Rio Grande (FURG), Pelotas, Rio Grande, Rio Grande do Sul, Brasil
  • João Batista da Silva Maciel Universidade Federal do Rio Grande (FURG), Pelotas, Rio Grande, Rio Grande do Sul, Brasil
  • Carla Vitola Gonçalves Universidade Federal do Rio Grande (FURG), Pelotas, Rio Grande, Rio Grande do Sul, Brasil
  • Andrea von Groll Universidade Federal do Rio Grande (FURG), Pelotas, Rio Grande, Rio Grande do Sul, Brasil
  • Odir Antônio Dellagostin Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil
  • Pedro Eduardo Almeida da Silva Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil
Keywords: Helicobacter pylori, genetic polymorphism, Interleukin-1 gene, cagA gene, gastric diseases

Abstract

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases.

Methodology: Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR.

Results: It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B.  No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found.

Conclusions: We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.

Published
2015-09-29
How to Cite
1.
Ramis I, Vianna J, Halicki PC, Lara C, Tadiotto T, da Silva Maciel J, Gonçalves C, von Groll A, Dellagostin O, da Silva PE (2015) Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis. J Infect Dev Ctries 9:1108-1116. doi: https://doi.org/10.3855/jidc.6123
Section
Original Articles