Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

  • Ricardo S. Yamatogi School of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
  • Heverton C. Oliveira School of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
  • Carlos H. Camargo Adolfo Lutz Institute. São Paulo, SP, Brazil
  • Sueli A. Fernandes Adolfo Lutz Institute. São Paulo, SP, Brazil
  • Rodrigo T. Hernandes School of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
  • José PAN Pinto School of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
  • Vera LM Rall Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
  • João P. Araújo Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, Brazil
Keywords: Poultry, Corvallis, Salmonella, ESBL, PFGE, fluoroquinolones

Abstract

Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains.

Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE).

Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype.

Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants.

Author Biography

Carlos H. Camargo, Adolfo Lutz Institute. São Paulo, SP, Brazil
Bacteriology Center, Scientific Researcher
Published
2015-10-29
How to Cite
1.
Yamatogi R, Oliveira H, Camargo C, Fernandes S, Hernandes R, Pinto J, Rall V, Araújo J (2015) Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse. J Infect Dev Ctries 9:1161-1165. doi: https://doi.org/10.3855/jidc.5634
Section
Brief Original Articles