Genotyping and prevalence of Chlamydia trachomatis infection among women in Belém, Pará, northern Brazil

  • Danielle M Brasiliense Instituto Evandro Chagas, Laboratório de Infecções Respiratórias de Origem Bacteriana, Ananindeua, Pará, Brasil
  • Bárbara N Borges Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Pará, Brasil
  • Wallax A S Ferreira Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Pará, Brasil
Keywords: C. trachomatis, genotyping, ompA gene

Abstract

Introduction: Genital Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases in women, and undetected cases of the disease are highly associated with long-term complications. Despite the high prevalence of infections in Brazil, very little is known about the distribution of C. trachomatis genovars. In this study, we determined the prevalence and genotypes of C. trachomatis in women treated at a public hospital in the Brazilian city of Belém, the capital of the state of Pará.

Methodology: A total of 154 women were tested for chlamydial infection by PCR using specific primers for the C. trachomatis cryptic plasmid. Genotyping of positive samples was performed by sequencing the ompA gene and conducting further phylogenetic analysis.

Results: Out of the 154 samples, 17 were found to be positive using C. trachomatis cryptic plasmid PCR. The overall prevalence of C. trachomatis infection was 11%, with the highest prevalence observed in women between 16 and 20 years of age. Five genotypes were found to be associated with endocervical infection. Genotype F was most frequently found (37.5%), followed by genotypes J (25%), E (25%), I (6.25%), and D (6.25%).

Conclusions: This study emphasizes the relevance of C. trachomatis infection in the young female population of the Brazilian Amazon region. It also demonstrates the diversity of genotypes involved in genital infection in this population.

Author Biography

Danielle M Brasiliense, Instituto Evandro Chagas, Laboratório de Infecções Respiratórias de Origem Bacteriana, Ananindeua, Pará, Brasil
Seção de Bacteriologia e Micologia
Published
2016-02-28
How to Cite
1.
Brasiliense DM, Borges BN, Ferreira WAS (2016) Genotyping and prevalence of Chlamydia trachomatis infection among women in Belém, Pará, northern Brazil. J Infect Dev Ctries 10:134-137. doi: 10.3855/jidc.6474
Section
Original Articles