Genetic characteristics of coxsackievirus A16 associated with hand, foot, and mouth disease in Nanjing, China

Wei Yong; Mengkai Qiao; Limin Shi; Xuan Wang; Yan Wang; Xuefei Du; Min He; Jie Ding

doi:10.3855/jidc.6876

Genetic characteristics of coxsackievirus A16 associated with hand, foot, and mouth disease in Nanjing, China

Authors

Wei Yong Nanjing Center for Disease Control and Prevention, Nanjing, China
Mengkai Qiao Nanjing Center for Disease Control and Prevention, Nanjing, China
Limin Shi Nanjing Center for Disease Control and Prevention, Nanjing, China
Xuan Wang Nanjing Center for Disease Control and Prevention, Nanjing, China
Yan Wang Nanjing Center for Disease Control and Prevention, Nanjing, China
Xuefei Du Nanjing Center for Disease Control and Prevention, Nanjing, China
Min He Nanjing Center for Disease Control and Prevention, Nanjing, China
Jie Ding Nanjing Center for Disease Control and Prevention, Nanjing, China

DOI:

https://doi.org/10.3855/jidc.6876

Keywords:

Coxsackievirus A16, phylogenetic analysis, VP1, antigenic position

Abstract

Introduction: Coxsackievirus A16 (CVA16) is a main pathogen in hand, foot, and mouth disease (HFMD) worldwide. This study intended to clarify the genetic characteristics of CVA16 associated with HFMD in a defined area in Nanjing, China.

Methodology: A total of 175 CVA16 strains isolated from throat swabs between 2011 and 2013 were obtained through sentinel hospitals in Nanjing. Multiplex polymerase chain reaction (PCR) was used to amplify the VP1 sequence of local CVA16 strains, and their genetic relationship with 138 CVA16 strains isolated in China and other countries of the world was compared.

Results: Phylogenetic analysis based on complete VP1 sequences revealed that subgenotype B1a and B1b were predominantly circulating in Nanjing and B1b strains were spread more widely. The evolution of CVA16 strains is very conservative, with a mean distance of less than 9%. Moreover, six reported conservative regions in VP1 protein were examined, and three of them exhibited high conservation in all CVA16 genotypes except the G-10 prototype and may serve for further vaccine research.

Conclusions: The CVA16 strains circulating in Nanjing, China, in 2011 to 2013 belonged to different genotypes and evolved in a conservative way. To provide further evidence for epidemiological linkage and evolutionary recombination events in CVA16, persistent surveillance of HFMD-associated pathogens is required.

Author Biographies

Wei Yong, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Mengkai Qiao, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Limin Shi, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Xuan Wang, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Yan Wang, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Xuefei Du, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Min He, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

Jie Ding, Nanjing Center for Disease Control and Prevention, Nanjing, China

Department of Microbiology

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Published

2016-02-28

How to Cite

Yong W, Qiao M, Shi L, Wang X, Wang Y, Du X, He M, Ding J (2016) Genetic characteristics of coxsackievirus A16 associated with hand, foot, and mouth disease in Nanjing, China. J Infect Dev Ctries 10:168–175. doi: 10.3855/jidc.6876

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Issue

Vol. 10 No. 02: February 2016

Section

Original Articles

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