New Delhi metallo-β-lactamase in Jamaica

  • Camille-Ann Thoms-Rodriguez University of West Indies, Kingston, Jamaica
  • Tony Mazzulli Mount Sinai Hospital/University Health Network, Department of Microbiology, Toronto, Ontario, Canada
  • Nicole Christian University of West Indies, Kingston, Jamaica
  • Barbara M Willey Mount Sinai Hospital/University Health Network, Department of Microbiology, Toronto, Ontario, Canada
  • David A Boyd Public Health Agency of Canada, Winnipeg, Manitoba, Canada
  • Laura F Mataseje Public Health Agency of Canada, Winnipeg, Manitoba, Canada
  • Michael R Mulvey Public Health Agency of Canada, Winnipeg, Manitoba, Canada
  • Celia DC Christie University of West Indies, Kingston, Jamaica
  • Alison M Nicholson University of West Indies, Kingston, Jamaica
Keywords: multidrug-resistant, organisms, NDM, Jamaica

Abstract

Introduction: The global dissemination of the New Delhi metallo-beta-lactamase (NDM) gene among certain strains of bacteria has serious implications since the infections caused by such organisms pose a therapeutic challenge. Although the NDM gene has been detected in various parts of the world, this is the first report of its detection in the English-speaking Caribbean. The NDM producing Klebsiella pneumoniae was isolated from an Indian patient who had recently relocated to Jamaica.

Methodology: Identification and susceptibility testing of the K. pneumoniae isolate was performed using the Vitek 2 automated system) in keeping with Clinical and Laboratory Standards Institute (CLSI) standards. It was identified as a metallobetalactamase producer using the Rosco KPC+MBL kit. Genotypic screening for common betalactamase (including carbapenemase) genes, was carried out  using two multiplex PCRs: one for SHV-, TEM-, CTX-M-, OXA-1-, and CMY-2-types, and one for VIM-, KPC-, IMP-, OXA-48, GES-, and NDM-types. Strain typing was conducted by pulsed-field gel electrophoresis (PFGE) using XbaI and multi-locus sequencing (MLS). Plasmid isolation and analysis was also performed.

Results: K. pneumoniae (N11-02395), not previously associated with the dissemination of the NDM in India, Sweden or the UK, was found to harbor the NDM-1 gene on plasmid pNDM112395.

Conclusion: The identification of the NDM-1 gene underscores the need for effective surveillance and infection control measures to identify and prevent spread of multidrug resistant Gram negative bacilli. Strict infection control measures implemented for this patient helped to prevent the spread of this organism to other patients.

Author Biography

Alison M Nicholson, University of West Indies, Kingston, Jamaica
Department of Microbiology Senior Lecturer Consultant Microbiologist
Published
2016-02-28
How to Cite
1.
Thoms-Rodriguez C-A, Mazzulli T, Christian N, Willey BM, Boyd DA, Mataseje LF, Mulvey MR, Christie CD, Nicholson AM (2016) New Delhi metallo-β-lactamase in Jamaica. J Infect Dev Ctries 10:183-187. doi: 10.3855/jidc.7094
Section
Brief Original Articles