Antimicrobial effect of probiotics on bacterial species from dental plaque

  • Csilla Zambori National Institute of Research – Development in the Pathology Domain and Biomedical Sciences “Victor Babes”, Bucharest, Romania
  • Attila Alexandru Morvay National Institute of Research – Development in the Pathology Domain and Biomedical Sciences “Victor Babes”, Bucharest, Romania
  • Claudia Sala Banat’s University of Agricultural Sciences and Veterinary Medicine, King Michael I of Romania, Timisoara, Romania
  • Monica Licker Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
  • Camelia Gurban Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
  • Gabriela Tanasie Victor Babes University of Medicine and Pharmacy, Timisoara, Romania
  • Emil Tirziu Banat’s University of Agricultural Sciences and Veterinary Medicine, King Michael I of Romania, Timisoara, Romania
Keywords: Antimicrobial, biofilm, dental plaque, oral cavity, probiotics

Abstract

Introduction: The antimicrobial role of probiotic Lactobacillus casei subspecies casei DG (L. casei DG) and of the mix culture of probiotic Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12 was tested on species of Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera from supragingival sites from dogs with dental disease of different breed, age, sex, weight, and diet. The research was conducted on these four genera because of their importance in zoonotic infections after dog bites.

Methodology: Species from Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera were isolated and identified. To test the antimicrobial efficacy of L. casei DG and the mixed culture of probiotic L. acidophilus LA-5 and Bifidobacterium bifidum BB-12 on the pathogenic species, the agar overlay method was used.

Results: L. casei DG had a bactericidal effect on all analyzed species isolated from Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera after 24 hours of incubation. The mixed probiotic culture made up of L. acidophilus LA-5 and Bifidobacterium BB-12 species had no bactericidal effect on the species of Staphylococcus and Streptococcus genera, which were resistant. However, it had a bacteriostatic effect on several species of Pasteurella and Neisseria genera.

Conclusions: This work highlights the antimicrobial potential of probiotics in vitro, demonstrating that the probiotic L. casei DG has a bactericidal effect on all analyzed species isolated from dental plaque and that the mix culture of probiotic L. acidophilus LA-5 and Bifidobacterium BB-12 has only a bacteriostatic effect.

Author Biographies

Csilla Zambori, National Institute of Research – Development in the Pathology Domain and Biomedical Sciences “Victor Babes”, Bucharest, Romania

Emergency County Hospital, Timisoara; Regional Center of Transplant and Immunology;

Assistant, DVM, PhD.

Attila Alexandru Morvay, National Institute of Research – Development in the Pathology Domain and Biomedical Sciences “Victor Babes”, Bucharest, Romania

Emergency County Hospital, Timisoara; Regional Center of Transplant and Immunology;

DVM, PhD.

Claudia Sala, Banat’s University of Agricultural Sciences and Veterinary Medicine, King Michael I of Romania, Timisoara, Romania

Department of Sanitary veterinary control of food from animal origin;

Associate Professor, DVM, PhD.

Monica Licker, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania

Department of Microbiology;

Professor, DM, PhD.

Camelia Gurban, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania

Deparment of Biochemistry;

Assistant, DM, PhD.

Gabriela Tanasie, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania

Department of Physiology and Immunology;

Associate Professor, DM, PhD.

Emil Tirziu, Banat’s University of Agricultural Sciences and Veterinary Medicine, King Michael I of Romania, Timisoara, Romania

Head of Deparment of Microbiology and Immunology;

Professor, DVM, PhD.

Published
2016-03-31
How to Cite
1.
Zambori C, Morvay AA, Sala C, Licker M, Gurban C, Tanasie G, Tirziu E (2016) Antimicrobial effect of probiotics on bacterial species from dental plaque. J Infect Dev Ctries 10:214-221. doi: 10.3855/jidc.6800
Section
Original Articles