Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey

  • Feyza Alp Selcuk University, Faculty of Medicine, Konya, Turkey
  • Duygu Findik Selcuk University, Faculty of Medicine, Konya, Turkey
  • Hatice Turk Dagi Selcuk University, Faculty of Medicine, Konya, Turkey
  • Ugur Arslan Selcuk University, Faculty of Medicine, Konya, Turkey
  • Aybike Tazegul Pekin Selcuk University, Faculty of Medicine, Konya, Turkey
  • Setenay Arzu Yilmaz Selcuk University, Faculty of Medicine, Konya, Turkey
Keywords: Group B streptococcus, carriage, culture, PCR, GBS antigen test, PFGE

Abstract

Introduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates.

Methodology: Recto-vaginal swab specimens were obtained from 500 women, and a questionnaire was administered to each to assess their risk factors for GBS carriage. A culture, GBS antigen test, and polymerase chain reaction (PCR) were performed on all samples. Antibiotic susceptibility testing was performed, and the clonal relationship was determined by pulsed-field gel electrophoresis (PFGE) on all viable isolates.

Results: Of the 500 women, sixty-eight (13.6%) women were GBS carriers, of whom 9.8% were pregnant and 16.5% not. There was a significant difference between GBS carriage and history of premature rupture of membrane (PROM). GBS was isolated from 65 (13%) samples. GBS was positive in 70 (14%) samples by antigen test and in 62 (12.4%) by PCR. Sixty-eight of the 70 positive antigen tests were confirmed by PCR or culture. Fifty-five isolates were resistant to tetracycline, 16 to erythromycin and clindamycin, and 13 to levofloxacin. Thirteen different pulsotypes and 17 sporadic strains were determined by PFGE.

Conclusions: GBS carriage rate in non-pregnant women was higher than in pregnant women. The GBS antigen test was more sensitive than culture and PCR. GBS isolates did not originate from a single clone and contained sporadic strains. There was a significant difference between GBS carriage and history of PROM. Epidemiologic data obtained in this study will help future studies.

Author Biographies

Feyza Alp, Selcuk University, Faculty of Medicine, Konya, Turkey
Microbiology
Duygu Findik, Selcuk University, Faculty of Medicine, Konya, Turkey
Microbiology
Hatice Turk Dagi, Selcuk University, Faculty of Medicine, Konya, Turkey
Microbiology
Ugur Arslan, Selcuk University, Faculty of Medicine, Konya, Turkey
Microbiology
Aybike Tazegul Pekin, Selcuk University, Faculty of Medicine, Konya, Turkey
Gynecology and Obstetrics
Setenay Arzu Yilmaz, Selcuk University, Faculty of Medicine, Konya, Turkey
Gynecology and Obstetrics
Published
2016-03-31
How to Cite
1.
Alp F, Findik D, Dagi HT, Arslan U, Pekin AT, Yilmaz SA (2016) Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey. J Infect Dev Ctries 10:222-226. doi: 10.3855/jidc.6190
Section
Original Articles