Role of Chlamydia pneumoniae in community-acquired pneumonia in hospitalized Jordanian adults

  • Suzan N Al-Aydie Faculty of Science, the University of Jordan, Amman, Jordan
  • Nathir M Obeidat Faculty of Medicine, the University of Jordan, Amman, Jordan
  • Hesham M Al-Younes Faculty of Medicine, the University of Jordan, Amman, Jordan
Keywords: Chlamydia pneumoniae, Jordan, microimmunofluorescence, PCR, pneumonia, respiratory disease

Abstract

Introduction: This study investigated the role of Chlamydia pneumoniae in the etiology of community-acquired pneumonia (CAP) in Jordanian adults.

Methodology: Eighty hospitalized CAP patients and 110 healthy adults were enrolled. Overall prevalences of C. pneumoniae IgG antibodies in sera and the rate of acute infection were estimated, using the microimmunofluorescence method (MIF), at titers of 1:16 and 1:512, respectively. Moreover, a nested polymerase chain reaction (PCR) was used to detect C. pneumoniae DNA in nasopharyngeal and blood Buffy coat samples.

Results: Overall chlamydial IgG prevalence was higher in CAP cases than controls (70% versus 61.8%). Similarly, higher rate of acute infection was found in patients than in controls (16.3% versus 5.5%). By focusing on subjects testing positive at 1:16, acute infection was detectable in 23.2% of CAP cases, compared with 8.8% of seropositive controls. Chlamydial DNA was confirmed in 8.2% and 8.8% of nasopharyngeal specimens from controls and patients, respectively. Moreover, 10.9% and 7.5% of Buffy coats from controls and cases, respectively, were PCR-positive. When performances of both assays for detection of the pathogen were assessed, the sensitivities of MIF and PCR were low and comparable. However, MIF demonstrated higher specificity, positive predictive value, and negative predictive value than PCR.

Conclusions: MIF-based data indicate that C. pneumoniae could be a potential causal agent of CAP in Jordan. This study may serve as a basis to elucidate the exact role C. pneumoniae and other co-infecting pathogens in the etiology of respiratory tract disease.

Author Biographies

Suzan N Al-Aydie, Faculty of Science, the University of Jordan, Amman, Jordan

Department of Biological Sciences
Faculty of Science

MsC Biology

 

Nathir M Obeidat, Faculty of Medicine, the University of Jordan, Amman, Jordan

Respiratory and Sleep Medicine

Professor
Hesham M Al-Younes, Faculty of Medicine, the University of Jordan, Amman, Jordan

Department of Biological Sciences
Faculty of Science

Associate Professor

 


Published
2016-03-31
How to Cite
1.
Al-Aydie SN, Obeidat NM, Al-Younes HM (2016) Role of Chlamydia pneumoniae in community-acquired pneumonia in hospitalized Jordanian adults. J Infect Dev Ctries 10:227-236. doi: 10.3855/jidc.6590
Section
Original Articles