Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing

Authors

  • Rajeswari Shome ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India
  • Natesan Krithiga ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India
  • Padmashree B Shankaranarayana ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India
  • Sankarasubramanian Jegadesan School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India
  • Vishnu Udayakumar S School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India
  • Bibek Ranjan Shome ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India
  • Girin Kumar Saikia College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India
  • Narendra Kumar Sharma Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
  • Harshad Chauhan College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India
  • Bharat Singh Chandel College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India
  • Rajendhran Jeyaprakash School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India
  • Habibur Rahman ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

DOI:

https://doi.org/10.3855/jidc.6617

Keywords:

Brucella, AMOS PCR, Bruce PCR, Indian isolates, MLST, qPCR

Abstract

Introduction: Brucellosis  is one of the most important zoonotic diseases that affects  multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country.

Methodology: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST).

Results: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14.

Conclusion: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.

Author Biographies

Rajeswari Shome, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

Bacteriology Section

Natesan Krithiga, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

Bacteriology Section

Padmashree B Shankaranarayana, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

Bacteriology Section

Sankarasubramanian Jegadesan, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India

Dept of Genetics

Vishnu Udayakumar S, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India

Dept of genetics

Bibek Ranjan Shome, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

Bacteriology Section

Girin Kumar Saikia, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam, India


Dept. of Microbiology, College of Veterinary Sciences, Assam Agricultural University

Narendra Kumar Sharma, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

Senior Bacteriologist cum Head
Deptt of Veterinay Microbiology
Guru Angad Dev Veterinary and Animal Sciences University

Harshad Chauhan, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India


Dept. of Animal Biotechnology, College of Veterinary Science and Animal Husbandry

Bharat Singh Chandel, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India


Dept. of Animal Biotechnology, College of Veterinary Science and Animal Husbandry,
Sardarkrushinagar Dantiwada Agricultural University

Rajendhran Jeyaprakash, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India


Dept. of Genetics, School of Biological Sciences, Madurai Kamaraj University

Habibur Rahman, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics (ICAR - NIVEDI) (Formerly PD_ADMAS),Yelahanka, Bengaluru, Karnataka, India

Bacteriology section

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Published

2016-03-31

How to Cite

1.
Shome R, Krithiga N, Shankaranarayana PB, Jegadesan S, Udayakumar S V, Shome BR, Saikia GK, Sharma NK, Chauhan H, Chandel BS, Jeyaprakash R, Rahman H (2016) Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing. J Infect Dev Ctries 10:237–244. doi: 10.3855/jidc.6617

Issue

Vol. 10 No. 03: March 2016

Section

Original Articles

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