Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro

  • Yan Tu Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
  • Yadong Chen Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
  • Chongyang Zheng The First People's Hospital affiliated to Huzhou Teachers' College, Huzhou, Zhejiang, China
  • Hui Chen Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
Keywords: S. sanguinis, P. gingivalis, Listerine antiseptic mouth rinse, Xipayi mouth rinse, platelet aggregation

Abstract

Introduction: The purpose of this study was to observe platelet aggregation promoted by biofilms of Streptococcus sanguinis and Porphyromonas gingivalis and to evaluate the effect of two different mouth rinses on this process.

Methodology: In the first experiment, the same amount of S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solution was added to an equivalent amount of platelet-rich plasma (PRP). Aggregation was measured using a recording platelet aggregometer.

In the second experiment, S. sanguinis, P. gingivalis, S sanguinis + P. gingivalis mixed solutions were pretreated with either Listerine antiseptic mouth rinse or Xipayi mouth rinse for 3 minutes, 6 minutes, and 10 minutes, respectively. The same amount of solution was added to the PRP, and the inhibition of aggregation was measured.

Results: In the first experiment, S. sanguinis and P. gingivalis were able to induce platelet aggregation. The aggregation rate of S. sanguinis + P. gingivalis was significantly lower than that of either S. sanguinis or P. gingivalis.

In the second experiment, when S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solutions were pretreated with Listerine antiseptic mouth rinse for 3 minutes and Xipayi mouth rinse for 10 minutes, there was no significant platelet aggregation.

Conclusions: Platelets could adhere to S. sanguinis or P. gingivalis, but when S. sanguinis was mixed with P. gingivalis, the aggregation rate was reduced significantly. Treatment with Listerine antiseptic mouth rinse or Xipayi mouth rinse inhibited the ability of the bacteria to induce platelet aggregation.

Author Biographies

Yan Tu, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
Department of conservative Dentistry and periodontics , Affiliated Hospital of Stomatology, Medical College
Yadong Chen, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
Department of conservative Dentistry and periodontics , Affiliated Hospital of Stomatology, Medical College
Chongyang Zheng, The First People's Hospital affiliated to Huzhou Teachers' College, Huzhou, Zhejiang, China
Department of Stomotology
Hui Chen, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, Zhejiang, China
Department of conservative Dentistry and periodontics , Affiliated Hospital of Stomatology, Medical College
Published
2016-08-02
How to Cite
1.
Tu Y, Chen Y, Zheng C, Chen H (2016) Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro. J Infect Dev Ctries 10:704-711. doi: 10.3855/jidc.6639
Section
Original Articles