Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection

Authors

  • Huijuan Zhu School of Public Health, Medical College of Soochow University, Suzhou, China
  • Yiying Wang School of Public Health, Medical College of Soochow University, Suzhou, China
  • Lugang Yu Suzhou Industrial Park Centers for Disease Control and Prevention, Suzhou, China
  • Yunfang Xu Huai’an Fourth Hospital, Huai’an, China
  • Hui Zhou Suzhou Industrial Park Centers for Disease Control and Prevention, Suzhou, China
  • Yi Ding Suzhou Industrial Park Centers for Disease Control and Prevention, Suzhou, China
  • Aili Wang School of Public Health, Medical College of Soochow University, Suzhou, China
  • Xingxiang Liu Huai’an Fourth Hospital, Huai’an, China
  • Chen Dong School of Public Health, Medical College of Soochow University, Suzhou, China

DOI:

https://doi.org/10.3855/jidc.7656

Keywords:

type 2 diabetes mellitus, hepatitis B virus, major hydrophilic region, mutation

Abstract

Introduction: This study aimed to further analyze the associations between type 2 diabetes mellitus (T2DM) and hepatitis B virus (HBV) infection, and to investigate the relationships between T2DM and the mutations within the HBV major hydrophilic region (MHR).

Methodology: In this cross-sectional study, 3,377 persons (338 T2DM patients and 3,039 non-diabetics) were randomly selected. HBsAg detection was performed by enzyme-linked immunosorbent assay. The HBV MHR was amplified, sequenced, and analyzed by nested PCR.

Results: The seroprevalence of HBsAg was 21.30% in T2DM patients (72/338), which was significantly higher than in non-diabetics (15.53%). Compared to persons without T2DM, the proportion of T2DM patients positive for HBsAg was significantly elevated in males, people > 55 years (p = 0.039), and people with a body mass index (BMI) ≥ 24 kg/m2. Totally, 112 genotype B and 111 genotype C HBV sequences were isolated. No significant difference in HBV genotype distribution was observed between T2DM patients and non-diabetics. Compared to genotype C HBV-infected cases in non-diabetics, the amino acid substitution rates in the MHR were significantly higher in T2DM patients (p = 0.003). Moreover, seven HBV strains with stop codon mutations within the HBV S gene were identified: three from T2DM patients (5.45%) and four from non-diabetics (2.38%).

Conclusions: In China, T2DM is significantly associated with chronic HBV infections and genotype C HBV MHR mutations. Being males, > 55 years of age, and ≥ 24 kg/m2 of BMI are the risk factors of HBV infection in T2DM patients.

Downloads

Published

2016-08-31

How to Cite

1.
Zhu H, Wang Y, Yu L, Xu Y, Zhou H, Ding Y, Wang A, Liu X, Dong C (2016) Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection. J Infect Dev Ctries 10:837–844. doi: 10.3855/jidc.7656

Issue

Vol. 10 No. 08: August 2016

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC