Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women

  • Daniele Maria Knupp de Souza Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Claudio Galuppo Diniz Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Didier Silveira Castellano Filho Faculdade de Ciências Médicas e da Saúde de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Laura Maria Andrade de Oliveira Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Débora Martins Coelho Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Luciana de Souza Talha Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Thiago César do Nascimento Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Alessandra Barbosa Ferreira-Machado Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Vânia Lúcia da Silva Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Keywords: bacterial vaginosis, Gardnerella vaginalis, Nugent score, antimicrobial resistance, clue-cells

Abstract

Introduction: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients.

Methodology: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined.

Results: Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%).

Conclusions: Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis.

Author Biography

Vânia Lúcia da Silva, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences
Published
2016-09-30
How to Cite
1.
Knupp de Souza DM, Diniz CG, Filho DSC, Andrade de Oliveira LM, Coelho DM, Talha L de S, Nascimento TC do, Ferreira-Machado AB, Silva VL da (2016) Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women. J Infect Dev Ctries 10:913-919. doi: 10.3855/jidc.7161
Section
Original Articles