Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria

  • Eyitayo O Adenipekun Olabisi Onabanjo University, College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria
  • Charlene R. Jackson USDA-ARS, Russell Research Center, Athens, GA, United States
  • Hazem Ramadan Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
  • Bamidele A Iwalokun College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria
  • Kolawole S Oyedeji College of Medicine, University of Lagos, Lagos, Nigeria
  • Jonathan G Frye USDA-ARS, Russell Research Center, Athens, GA, United States
  • John B Barrett USDA-ARS, Russell Research Center, Athens, GA, United States
  • Lari M Hiott USDA-ARS, Russell Research Center, Athens, GA, United States
  • Tiffanie A Woodley USDA-ARS, Russell Research Center, Athens, GA, United States
  • Afolabi Oluwadun Olabisi Onabanjo University, College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria
Keywords: E. coli, antimicrobial resistance, humans, Nigeria

Abstract

Introduction: The emergence of multidrug resistance (MDR; resistance to ≥ 2 more antimicrobials) in Escherichia coli is of concern due to complications encountered in treatment.

Methodology: In this study, prevalence, antimicrobial resistance, and genetic characteristics of MDR community isolates of E. coli from Lagos, Nigeria were determined. Urine and stool samples were obtained from outpatients attending Lagos State hospitals and from animal handlers in abattoirs, poultries, and open markets, from December 2012 to July 2013.

Results: Approximately 50% of urine (200/394) and 88% of stool samples (120/136) were positive for E. coli. Based upon β-lactamase production, a subset of those isolates was selected for further study. Of the 22 antimicrobials tested, E. coli exhibited resistance to all antimicrobials except amikacin and piperacillin/tazobactam. The highest levels of resistance were to tetracycline (182/247; 73.7%), trimethoprim/sulfamethoxazole (152/247; 61.5%), and ampicillin (147/247; 59.1%). Resistance to the cephalosporins ranged from 1.6%–15% including the third- and fourth-generation cephalosporins, cefpodoxime (20/247; 8.1%) and cefepime (4/247; 1.6%), respectively. MDR was observed in 69.6% (172/247) of the isolates. Forty-eight E. coli resistant to at least five antimicrobials were selected for further analysis using pulsed-field gel electrophoresis; seven distinct clusters were observed among the diverse patterns. Of the 48 MDR E. coli, 30 different sequence types (ST) were detected using multilocus sequence typing, including four ST131.

Conclusions: This study demonstrated circulating MDR E. coli in the Nigerian community. Monitoring of antimicrobial resistance in developing countries is necessary to optimize empiric treatment and the prudent use of antimicrobials.

Author Biographies

Eyitayo O Adenipekun, Olabisi Onabanjo University, College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria

Department of Medical Microbiology and Parasitology, College of Health Sciences

MS

Charlene R. Jackson, USDA-ARS, Russell Research Center, Athens, GA, United States

Bacterial Epidemiology and Antimicrobial Resistance Research Unit

MS, PhD

Hazem Ramadan, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Hygiene and Zoonoses Department

PhD

Bamidele A Iwalokun, College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria

Department of Biochemistry

PhD

Kolawole S Oyedeji, College of Medicine, University of Lagos, Lagos, Nigeria

Department of Medical Microbiology and Parasitology

PhD

Jonathan G Frye, USDA-ARS, Russell Research Center, Athens, GA, United States

Bacterial Epidemiology and Antimicrobial Resistance Research Unit

PhD

John B Barrett, USDA-ARS, Russell Research Center, Athens, GA, United States

Bacterial Epidemiology and Antimicrobial Resistance Research Unit

BS

Lari M Hiott, USDA-ARS, Russell Research Center, Athens, GA, United States

Bacterial Epidemiology and Antimicrobial Resistance Research Unit

MS

Tiffanie A Woodley, USDA-ARS, Russell Research Center, Athens, GA, United States

Bacterial Epidemiology and Antimicrobial Resistance Research Unit

BS

Afolabi Oluwadun, Olabisi Onabanjo University, College of Health Sciences, Sagamu campus, Sagamu, Ogun-State, Nigeria
Department of Medical Microbiology and Parasitology
Published
2016-09-30
How to Cite
1.
Adenipekun EO, Jackson CR, Ramadan H, Iwalokun BA, Oyedeji KS, Frye JG, Barrett JB, Hiott LM, Woodley TA, Oluwadun A (2016) Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria. J Infect Dev Ctries 10:920-931. doi: 10.3855/jidc.7997
Section
Original Articles