Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

  • Huong Thi Thanh Doan Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Xuyen Thi Kim Le Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Roan Thi Do Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Chau Thi Minh Hoang Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Khue Thi Nguyen Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Thanh Hoa Le Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Keywords: duck hepatitis A virus, VP1, genotyping, inter/intragenotypic variation, Vietnam

Abstract

Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013.

Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed.

Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%–100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%–100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains.

Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam.

Author Biographies

Huong Thi Thanh Doan, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Department of Immunology
Xuyen Thi Kim Le, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Department of Immunology
Thanh Hoa Le, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Department of Immunology
Published
2016-09-30
How to Cite
1.
Doan HTT, Le XTK, Do RT, Hoang CTM, Nguyen KT, Le TH (2016) Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam. J Infect Dev Ctries 10:988-995. doi: 10.3855/jidc.7239
Section
Original Articles