Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire

Valerio Iebba; Floriana Santangelo; Valentina Totino; Fabrizio Pantanella; Anatole Monsia; Veronica Di Cristanziano; David Di Cave; Serena Schippa; Federica Berrilli; Rossella D'Alfonso

doi:10.3855/jidc.8179

Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire

Authors

Valerio Iebba Sapienza University of Rome, Rome, Italy
Floriana Santangelo Sapienza University of Rome, Rome, Italy
Valentina Totino Sapienza University of Rome, Rome, Italy
Fabrizio Pantanella Sapienza University of Rome, Rome, Italy
Anatole Monsia Centre Don Orione pour handicapés physiques, Bonoua, Côte d’Ivoire
Veronica Di Cristanziano University of Cologne, Cologne, Germany
David Di Cave University of Rome Tor Vergata, Rome, Italy
Serena Schippa Sapienza University of Rome, Rome, Italy
Federica Berrilli University of Rome Tor Vergata, Rome, Italy
Rossella D'Alfonso Centre Don Orione pour handicapés physiques, Bonoua, Côte d’Ivoire

DOI:

https://doi.org/10.3855/jidc.8179

Keywords:

microbiota, Giardia duodenalis, Entamoeba spp., Blastocystis hominis, dysbiosis, Côte d’Ivoire

Abstract

Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites.

Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Côte d’Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data.

Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4×10^-6). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio.

Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.

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Published

2016-09-30

How to Cite

Iebba V, Santangelo F, Totino V, Pantanella F, Monsia A, Di Cristanziano V, Di Cave D, Schippa S, Berrilli F, D’Alfonso R (2016) Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire. J Infect Dev Ctries 10:1035–1041. doi: 10.3855/jidc.8179

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Issue

Vol. 10 No. 09: September 2016

Section

Brief Original Articles

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