Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates

  • Yun Mei Lai Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
  • Myo Thura Zaw Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
  • Shamsul Bahari Shamsudin Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
  • Zaw Lin Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
Keywords: usp gene, uropathogenic Escherichia coli, PAIusp subtyping, characterization

Abstract

Introduction: Uropathogenic virulence factors have been identified by comparing the prevalence of these among urinary tract isolates and environmental strains. The uropathogenic-specific protein (USP) gene is present on the pathogenicity island (PAI) of uropathogenic Escherichia coli (UPEC) and, depending on its two diverse gene types and the sequential patterns of three open reading frame units (orfUs) following it, there is a method to characterize UPEC epidemiologically called PAIusp subtyping.

Methodology: A total of 162 UPEC isolates from Sabah, Malaysia, were tested for the presence of the usp gene and the sequential patterns of three orfUs following it using polymerase chain reaction (PCR). In addition, by means of triplex PCR, the prevalence of the usp gene was compared with other two VFs of UPEC, namely alpha hemolysin (α-hly) and cytotoxic necrotizing factor (cnf-1) genes encoding two toxins.

Results: The results showed that the usp gene was found in 78.40% of UPEC isolates, indicating that its prevalence was comparable to that found in a previous study in Japan. The two or three orfUs were also associated with the usp gene in this study. All the PAIusp subtypes observed in Japan were present in this study, while subtype IIa was the most common in both studies. The usp gene was observed in a higher percentage of isolates when compared with ɑ-hly and cnf-1 genes.

Conclusions: The findings in Japan and Sabah, East Malaysia, were similar, indicating that PAIusp subtyping is applicable to the characterization of UPEC strains epidemiologically elsewhere in the world.

Author Biographies

Yun Mei Lai, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia

Department of Pathobiological and Medical Diagnostics.

Ph.D. student

 

Myo Thura Zaw, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia

Department of Pathobiological and Medical Diagnostics.

Senior Lecturer

Shamsul Bahari Shamsudin, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia

Department of Community and Family Medicine.

Associate Professor

Zaw Lin, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia

Department of Pathobiological and Medical Diagnostics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah.

Associate Professor

Published
2016-10-31
How to Cite
1.
Lai YM, Zaw MT, Shamsudin SB, Lin Z (2016) Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates. J Infect Dev Ctries 10:1053-1058. doi: 10.3855/jidc.6944
Section
Original Articles