Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali

  • Samba Adama Sangare Gabriel Touré University Teaching Hospital, Bamako, Mali
  • Almoustapha Issiaka Maiga Gabriel Touré University Teaching Hospital, Bamako, Mali
  • Ibrehima Guindo Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali
  • Aminata Maiga Point G University Teaching Hospital, Bamako, Mali
  • Namory Camara Gabriel Touré University Teaching Hospital, Bamako, Mali
  • Oumar Agaly Dicko Point G University Teaching Hospital, Bamako, Mali
  • Souleymane Diallo Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali
  • Flabou Bougoudogo Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali
  • Laurence Armand-Lefevre Bichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, France
  • Antoine Andremont Bichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, France
  • Ibrahim Izetiegouma Maiga Point G University Teaching Hospital, Bamako, Mali
Keywords: Enterobacteriaceae, extended-spectrum beta-lactamases, blood cultures, Bamako, Mali

Abstract

Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals of Bamako, Mali.

Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST).

Results: The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae.

Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively).

Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.

Author Biographies

Samba Adama Sangare, Gabriel Touré University Teaching Hospital, Bamako, Mali

PharmD, MSc, PhD Student

Almoustapha Issiaka Maiga, Gabriel Touré University Teaching Hospital, Bamako, Mali

PharmD, PhD

Ibrehima Guindo, Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali

PharmD, MSc, PhD Student

Aminata Maiga, Point G University Teaching Hospital, Bamako, Mali

PharmD, MSc, PhD Student

Namory Camara, Gabriel Touré University Teaching Hospital, Bamako, Mali

PharmD, MSc Student

Oumar Agaly Dicko, Point G University Teaching Hospital, Bamako, Mali

PharmD, MSc Student

Souleymane Diallo, Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali

PharmD, PhD, PU-PH

Flabou Bougoudogo, Faculty of Pharmacy, University of Sciences, Techniques, and Technologies of Bamako (USTTB), Bamako, Mali

PharmD, PhD, PU-PH

 

Laurence Armand-Lefevre, Bichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, France

PharmD, PhD, PH

Antoine Andremont, Bichat- Claude Bernard University Teaching Hospital and UMR INSERM 1137 Iame Paris, France

MD, PhD, PU-PH

Ibrahim Izetiegouma Maiga, Point G University Teaching Hospital, Bamako, Mali

MD, PhD, PU-PH

Published
2016-10-31
How to Cite
1.
Sangare SA, Maiga AI, Guindo I, Maiga A, Camara N, Dicko OA, Diallo S, Bougoudogo F, Armand-Lefevre L, Andremont A, Maiga II (2016) Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali. J Infect Dev Ctries 10:1059-1064. doi: 10.3855/jidc.7536
Section
Original Articles