Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

  • Ingrid Cécile Djuikoue Université des Montagnes, Bangangté, Cameroun
  • Paul-Louis Woerther Institut Gustave-Roussy, Villejuif, France
  • Michel Toukam Université des Montagnes, Bangangté, Cameroun
  • Charles Burdet Hôpital Bichat-Claude Bernard, AP-HP, Paris, France
  • Etienne Ruppé Hôpital Bichat-Claude Bernard, AP-HP, Paris, France
  • Kamga Hortense Gonsu Université de Yaoundé I, Yaoundé, Cameroun
  • Charles Fokunang Université de Yaoundé I, Yaoundé, Cameroun
  • Assiya El Mniai Hôpital Bichat-Claude Bernard, AP-HP, Paris, France
  • Kamgue Larissa Université de Yaoundé I, Yaoundé, Cameroun
  • Anatole Constant Pieme Université de Yaoundé I, Yaoundé, Cameroun
  • Mallila Georgia Mboupaing Université de Yaoundé I, Yaoundé, Cameroun
  • Caroline Mietchop Kakam Université de Yaoundé I, Yaoundé, Cameroun
  • Hervé Kengne Fogang Université de Yaoundé I, Yaoundé, Cameroun
  • Antoine Andremont Université Paris-Diderot, Paris, France
  • Jeanne Ngogang Université de Yaoundé I, Yaoundé, Cameroun
Keywords: ESBL, carriage, UTI, Cameroon, community

Abstract

Introduction: During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases – producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women.

Methodology: A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed.

Results: The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure.

Conclusions: Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.

Published
2016-10-31
How to Cite
1.
Djuikoue IC, Woerther P-L, Toukam M, Burdet C, Ruppé E, Gonsu KH, Fokunang C, El Mniai A, Larissa K, Pieme AC, Mboupaing MG, Kakam CM, Fogang HK, Andremont A, Ngogang J (2016) Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries 10:1135-1139. doi: 10.3855/jidc.7616
Section
Brief Original Articles