Efficacy of lytic Staphylococcus aureus bacteriophage against multidrug-resistant Staphylococcus aureus in mice

  • Joseph Michael Ochieng' Oduor Institute of Primate Research (IPR), Nairobi, Kenya
  • Nyamongo Onkoba Institute of Primate Research (IPR), Nairobi, Kenya
  • Fredrick Maloba School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya
  • Washingtone Ouma Arodi School of Medicine, Kenyatta University, Nairobi, Kenya
  • Atunga Nyachieo Institute of Primate Research (IPR), Nairobi, Kenya

Abstract

Introduction: The use of bacteriophages as an alternative treatment method against multidrug-resistant bacteria has not been explored in Kenya. This study sought to determine the efficacy of environmentally obtained lytic bacteriophage against multidrug-resistant Staphylococcus aureus (MDRSA) bacterium in mice.

Methodology: Staphylococcus aureus bacterium and S. aureus-specific lytic phage were isolated from sewage and wastewater collected within Nairobi County, Kenya. Thirty mice were randomly assigned into three groups: MDRSA infection group (n = 20), phage-infection group (n = 5), and non-infection group (n = 5). The MDRSA infection group was further subdivided into three groups: clindamycin treatment (8 mg/kg; n = 5), lytic phage treatment (108 PFU/mL (n = 5), and a combination treatment of clindamycin and lytic phage (n = 5). Treatments were done at either 24 or 72 hours post-infection (p.i), and data on efficacy, bacterial load, and animal physical health were collected.

Results: Treatment with phage was more effective (100%) than with clindamycin (62.25% at 24 hours p.i and 87.5% at 72 hours p.i.) or combination treatment (75% at 24 hours p.i. and 90% at 72 hours p.i.) (p < 0.001).

Conclusions: The results show that the environmentally obtained S. aureus lytic bacteriophage has therapeutic potential against MDRSA bacterium in mice.

Author Biographies

Joseph Michael Ochieng' Oduor, Institute of Primate Research (IPR), Nairobi, Kenya
Reproductive Health Biology Department - Institute of PrimateResearch.

Rank: Research Student

and

Medical Laboratory Science Department, School of Medicine- Kenyatta University.

Rank: Master's graduate student

Nyamongo Onkoba, Institute of Primate Research (IPR), Nairobi, Kenya

Tropical Infectious Diseases Department,

Rank: Senior Research Scientist at IPR

 

Fredrick Maloba, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya

Department of Zoological Sciences,

School of Pure and Applied  Sciences.

Rank: Lecturer

Washingtone Ouma Arodi, School of Medicine, Kenyatta University, Nairobi, Kenya

Medical Laboratory Science Department -School of Medicine,

Rank: Senior Lecturer.

 

Atunga Nyachieo, Institute of Primate Research (IPR), Nairobi, Kenya

Reproductive Health Biology Department.

Rank:Senior research Scientist at IPR

and

Department of Biochemistry, School of Medicine.

Rank: Senior Lecturer at UoN

Published
2016-11-24
How to Cite
Oduor J, Onkoba N, Maloba F, Arodi W, Nyachieo A (2016) Efficacy of lytic Staphylococcus aureus bacteriophage against multidrug-resistant Staphylococcus aureus in mice. The Journal Of Infection In Developing Countries 10 (11): 1208-1213. https://doi.org/10.3855/jidc.7931
Section
Original Articles

Keywords

MDRSA; efficacy; phage therapy; waste and sewage water