Comparison of HRM analysis and three REP-PCR genomic fingerprint methods for rapid typing of MRSA at a Brazilian hospital

  • Giovana Carolina Bodnar Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Heloísa Moreira Martins Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Caio Ferreira de Oliveira Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Alexandre Tadachi Morey Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Eliandro Reis Tavares Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Juscélio Donizete Cardoso Instituto Agronômico do Paraná, Londrina, PR, Brazil
  • Marcia Regina Eches Perugini Universidade Estadual de Londrina, Brazil
  • Lucy Megumi Yamauchi Lioni Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Sueli Fumie Yamada-Ogatta Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Renata Katsuko Takayama Kobayashi Biological Sciences Center, Universidade Estadual de Londrina, Brazil
  • Gerson Nakazato Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Keywords: antimicrobial, MRSA, genotypic profile, Staphylococcus aureus

Abstract

Introduction: Infections caused by multidrug-resistant bacteria are increasingly common and represent a serious problem for public health. Staphylococcus aureus is one of the major agents of infections, and methicillin-resistant S. aureus (MRSA) has spread worldwide. The aim of this study was to phenotypically and genotypically characterize 55 MRSAs isolated in the University Hospital of Londrina, Paraná, Brazil, during 2010.

Methodology: Bacterial isolates were characterized based on their antimicrobial susceptibility profile, biofilm production capacity, and staphylococcal chromosome cassette mec (SCCmec) type. Determination of clonal groups was performed by polymerase chain reaction using the RW3A, JB1, and BOX A1R primers and high-resolution melting (HRM) analysis.

Results: The majority of isolates harbored SCCmec type II. SCCmec III, characteristic of the Brazilian endemic clone, was observed in four strains. Only two isolates harbored SCCmec type IV, which is common in community-acquired MRSA strains. Most isolates also showed resistance to more than four of the tested antimicrobials, and 30 isolates exhibited the ability to produce biofilm. DNA polymorphism analysis showed a higher discriminatory power for the JB1 primer, but RW3A revealed several clonal groups of MRSA with similar genotypic and phenotypic characteristics. HRM analysis showed eight different sequence types.

Conclusions: These results are important for epidemiological studies involving MRSA infections.

Author Biographies

Giovana Carolina Bodnar, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Heloísa Moreira Martins, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Caio Ferreira de Oliveira, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Alexandre Tadachi Morey, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Programa Nacional de Pós Doutorado (PNPD/CAPES), Department of Microbiology, Biological Sciences Center
Eliandro Reis Tavares, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Juscélio Donizete Cardoso, Instituto Agronômico do Paraná, Londrina, PR, Brazil
Laboratory of Microbiology of Soil
Marcia Regina Eches Perugini, Universidade Estadual de Londrina, Brazil
Department of Pathology, Clinical and Toxicological Analysis
Lucy Megumi Yamauchi Lioni, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Sueli Fumie Yamada-Ogatta, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
1Department of Microbiology, Biological Sciences Center
Renata Katsuko Takayama Kobayashi, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Gerson Nakazato, Biological Sciences Center, Universidade Estadual de Londrina, Brazil
Department of Microbiology, Biological Sciences Center
Published
2016-12-30
How to Cite
1.
Bodnar GC, Martins HM, de Oliveira CF, Morey AT, Tavares ER, Cardoso JD, Perugini MRE, Lioni LMY, Yamada-Ogatta SF, Kobayashi RKT, Nakazato G (2016) Comparison of HRM analysis and three REP-PCR genomic fingerprint methods for rapid typing of MRSA at a Brazilian hospital. J Infect Dev Ctries 10:1306-1317. doi: 10.3855/jidc.7887
Section
Original Articles