Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai'an, China

Hongna Zhang; Xiaonan Zhao; Zhenzhen Zhai; Qing Li; Shuyuan Guo; Weishan Chang

doi:10.3855/jidc.8449

Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai'an, China

Authors

Hongna Zhang Hebei University of Ecomonics and Business, Shijiazhuang, China
Xiaonan Zhao Shandong Agricultural University, Tai'an, China
Zhenzhen Zhai Central Hospital of Tai'an City, Tai'an, China
Qing Li Shandong Agricultural University, Tai'an, China
Shuyuan Guo Shandong Agricultural University, Tai'an, China
Weishan Chang Shandong Agricultural University, Tai'an, China

DOI:

https://doi.org/10.3855/jidc.8449

Keywords:

Thermotolerant coliform, integron, water reservoir, ESBL, antibiotic resistance genes

Abstract

Introduction: The contamination of water environments by extended-spectrum β-lactamases-producing thermotolerant coliforms (ESBL-TC) has aroused public concern. However, little epidemiological data on ESBL-TC isolates from water reservoirs is available in China.

Methodology: This study was designed to investigate antibiotic resistance, bla gene types, and the presence of integrons (class 1, 2, and 3) and gene cassettes in ESBL-TC isolated from the Huangqian Reservoir of Tai'an, China.

Results: A total of 96 non-duplicate ESBL-TC were obtained in this study and the ESBL genes included bla_CTX-M-14(n = 47), bla_CTX-M-15(n = 27), bla_CTX-M-55(n = 18), bla_SHV-12(n = 4), bla_CTX-M-3(n = 3), and bla_CTX-M-123(n = 1). Eighty-three of the ninety-six ESBL-TC contained class 1 integrons (86.5%), and 2 isolates harbored class 2 integrons. The sizes of gene cassette regions within integrons were ranged from 0.2 kb to 3.2 kb.

Conclusions: The findings of this study indicated the widespread presence of ESBL-TC strains in the Huangqian Reservoir and spotlighted the potential role of water bodies as reservoirs for antibiotic resistant genes.

Author Biographies

Hongna Zhang, Hebei University of Ecomonics and Business, Shijiazhuang, China

Department of Teaching Management

Xiaonan Zhao, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Zhenzhen Zhai, Central Hospital of Tai'an City, Tai'an, China

Digestive Disease Institute

Qing Li, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Shuyuan Guo, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Weishan Chang, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Downloads

Published

2017-10-31

How to Cite

Zhang H, Zhao X, Zhai Z, Li Q, Guo S, Chang W (2017) Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai’an, China. J Infect Dev Ctries 11:740–746. doi: 10.3855/jidc.8449

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 11 No. 10: October 2017

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).