Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds

Camila B Rocha; Luz Alba A.M.G. Fornells; Miguel Rojas; Maíra Libetal; Alberto Manchego; Danilo Pezo; Norma Santos

doi:10.3855/jidc.9528

Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds

Authors

Camila B Rocha Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Luz Alba A.M.G. Fornells Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Miguel Rojas Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Maíra Libetal Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro, Niterói, RJ, Brazil
Alberto Manchego Universidad Nacional Mayor de San Marcos, (FMV/UNMSM), Lima, Peru
Danilo Pezo Instituto Veterinario de Investigaciones Tropicales y de Altura (IVITA), Cuzco, Peru
Norma Santos Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

DOI:

https://doi.org/10.3855/jidc.9528

Keywords:

Coronavirus, Bovine, Alpaca, Diarrhoea, Epidemiology

Abstract

Introduction: The enteric disorders represent a serious hazard for bovine and camelid breeding. The aim of this study was to examine the frequency of detection and molecular characteristics of enteric coronavirus (CoV) infections in cattle, alpaca, and llama herds bred in family-based farms in Brazil and Peru.

Methodology: Stool samples were collected from calves from Brazil and camelids from Peru for detection and characterization of CoV by reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis.

Results: 46.5% (47/101) samples from calves and 26.8% (70/261) from alpaca tested positive for CoV. All strains belong to lineage A1 of the Betacoronavirus genus. Phylogenetic analysis showed high identity between CoV strains detected in calves and alpacas.

Conclusions: This study characterised CoV strains from dairy cattle herds in the state of Rio de Janeiro, Brazil, and indicated that this virus is spread among the state herds. The results also indicate widespread circulation of CoV among the alpacas of Cuzco, Peru.

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Published

2018-01-31

How to Cite

Rocha CB, Fornells LAA, Rojas M, Libetal M, Manchego A, Pezo D, Santos N (2018) Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds. J Infect Dev Ctries 12:037–042. doi: 10.3855/jidc.9528

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Issue

Vol. 12 No. 01: January 2018

Section

Original Articles

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