Increase of blaOXA-23-like in Acinetobacter baumannii at a tertiary care center in Lebanon between 2007 and 2013

Souha S Kanj; Ralph Tayyar; Marwa Shehab; Bassam El-Hafi; Sari S Rasheed; Kohar Annie Kissoyan; Zeina A Kanafani; Rima Hanna Wakim; Nada Kara Zahreddine; George F Araj; Ghassan Dbaibo; Ghassan M Matar

doi:10.3855/jidc.10100

Increase of blaOXA-23-like in Acinetobacter baumannii at a tertiary care center in Lebanon between 2007 and 2013

Authors

Souha S Kanj Center for Infectious Diseases Research (CIDR), American University of Beirut Medical Center, Beirut, Lebanon
Ralph Tayyar Center for Infectious Diseases Research (CIDR), American University of Beirut Medical Center, Beirut, Lebanon
Marwa Shehab American University of Beirut
Bassam El-Hafi American University of Beirut https://orcid.org/0000-0003-3277-2948
Sari S Rasheed American University of Beirut
Kohar Annie Kissoyan American University of Beirut
Zeina A Kanafani American University of Beirut Medical Center
Rima Hanna Wakim American University of Beirut Medical Center
Nada Kara Zahreddine American University of Beirut Medical Center
George F Araj American University of Beirut Medical Center
Ghassan Dbaibo American University of Beirut Medical Center
Ghassan M Matar American University of Beirut

DOI:

https://doi.org/10.3855/jidc.10100

Keywords:

a. baumannii, oxa-23, rapd

Abstract

Introduction: The multi-drug resistant nature of Acinetobacter baumannii isolates have rendered many broad-spectrum antimicrobial agents ineffective against them. The purpose of this retrospective study is to define and compare the molecular characteristics of A. baumannii isolates from patients at a tertiary care center in Lebanon from two outbreaks, the first in 2007-2008, as part of a case-controlled study involving A. baumannii cases admitted to the ICU, and the second in 2013.

Methodology: A total of 148 A. baumannii clinical isolates were collected from various clinical specimens during 2007-2008 and 2013. All A. baumannii isolates were screened for bla_OXA-23-like and bla_OXA-51-likegenes of carbapenem resistance. Additionally, in an effort to assess the degree of the isolates’ genomic relatedness, random amplification of polymorphic DNA (RAPD) was performed.

Results: There was an increase in the prevalence of bla_OXA-23-like and bla_OXA-51-like genes between the two time periods; however, only 22% isolate genomic relatedness was calculated between 2007-2008 and 2013. Taking 80% as a margin of compatibility, 31 distinct clusters containing 2 to 11 strains were observed when both time periods were analyzed.

Conclusion: The presence of numerous clusters accompanied by a predominant increase in the prevalence of bla_OXA-23-like between 2007 and 2013 suggests a horizontal transmission of the gene within various strains of the species, contributing to the persistent increase in carbapenem resistance over the years. Therefore, infection control measures are required with compliance among all healthcare workers.

Author Biography

Ghassan M Matar, American University of Beirut

Professor Dept. of Microbiology and Immunology

Downloads

Published

2018-02-22

How to Cite

Kanj SS, Tayyar R, Shehab M, El-Hafi B, Rasheed SS, Kissoyan KA, Kanafani ZA, Hanna Wakim R, Kara Zahreddine N, Araj GF, Dbaibo G, Matar GM (2018) Increase of blaOXA-23-like in Acinetobacter baumannii at a tertiary care center in Lebanon between 2007 and 2013. J Infect Dev Ctries 12:4S. doi: 10.3855/jidc.10100

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 12 No. 02.1: February 2018 - SUPPLEMENT

Section

The Lebanese LSIDCM

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).