Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii

Mohsin Khurshid; Muhammad Hidayat Rasool; Muhammad Hussnain Siddique; Farrukh Azeem; Muhammad Naeem; Muhammad Sohail; Muhammad Sarfraz; Muhammad Saqalein; Zeeshan Taj; Muhammad Atif Nisar; Muhammad Usman Qamar; Asim Shahzad

doi:10.3855/jidc.11410

Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii

Authors

Mohsin Khurshid Department of Microbiology, Government College University, Faisalabad, Pakistan
Muhammad Hidayat Rasool Department of Microbiology, Government College University, Faisalabad, Pakistan
Muhammad Hussnain Siddique Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan
Farrukh Azeem Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan
Muhammad Naeem Department of Microbiology, Government College University, Faisalabad, Pakistan
Muhammad Sohail Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan
Muhammad Sarfraz Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan
Muhammad Saqalein Department of Microbiology, Government College University, Faisalabad, Pakistan
Zeeshan Taj Department of Microbiology, Government College University, Faisalabad, Pakistan
Muhammad Atif Nisar Department of Microbiology, Government College University, Faisalabad, Pakistan
Muhammad Usman Qamar Department of Microbiology, Government College University, Faisalabad, Pakistan
Asim Shahzad Department of Pathology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad, Pakistan

DOI:

https://doi.org/10.3855/jidc.11410

Keywords:

CRAB, surveillance, aminoglycosides, tetracyclines, sulfonamides

Abstract

Introduction: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene.

Methodology: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes.

Results: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC₉₀, 1 g/mL) Colistin (MIC₉₀, 1 g/mL) and Tigecycline (MIC₉₀, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations.

Conclusion: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management.

Author Biography

Mohsin Khurshid, Department of Microbiology, Government College University, Faisalabad, Pakistan

Lecturer, College of Allied Health Professionals, Directorate of Medical sciences, Government College University, Faisalabad, Pakistan

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Published

2019-10-31

How to Cite

Khurshid M, Rasool MH, Siddique MH, Azeem F, Naeem M, Sohail M, Sarfraz M, Saqalein M, Taj Z, Nisar MA, Qamar MU, Shahzad A (2019) Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii. J Infect Dev Ctries 13:899–905. doi: 10.3855/jidc.11410

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Issue

Vol. 13 No. 10: October 2019

Section

Original Articles

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