Antibiotic resistance profile of commensal Escherichia coli isolated from healthy sheep in Qatar

  • Nahla O Eltai Biomedical Research Center, Qatar University, Doha, Qatar
  • Asmaa A Al Thani Biomedical Research Center, Qatar University, Doha, Qatar
  • Sara H Al-Hadidi Biomedical Research Center, Qatar University, Doha, Qatar
  • Elmoubasher A Abdfarag Public Health Department, Ministry of Public Health, Doha, Qatar
  • Hamad Al-Romaihi Public Health Department, Ministry of Public Health, Doha, Qatar
  • Mahmoud H Mahmoud Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar
  • Osama K Alawad Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar
  • Hadi M Yassine Biomedical Research Center, Qatar University, Doha, Qatar
Keywords: Antibiotic resistance, Qatar, Sheep, E. coli

Abstract

Introduction: The uncontrolled antibiotics use in livestock is a leading factor for the emergence and spread of resistant bacteria from food animals to humans through the food chain. This study aims at evaluating the magnitude of the antimicrobial resistance (AMR) in food animals, acknowledging the lack of information on the prevalence of resistance in the veterinary field in Qatar.

Methodology: Rectal samples were collected from 171 sheep across three localities in Qatar between December 2016 and July 2017. These rectal samples were suspended in Phosphate buffer solution (PBS). Then streaked onto a selective CHROMagar E. coli medium plates and incubated at 37°C for 18 to 24 h. Isolated E. coli were tested for antibiotic susceptibility against 16 clinically- relevant antibiotics using the E-test method. Statistical analyses were performed using SPSS statistics 24.

Results: E. coli was isolated from 144 samples (84.2%), of which 90% were resistant to at least one antibiotic and 44% were multi-drug resistant (MDR). The highest resistance was against ciprofloxacin 69.4% (100), followed by nitrofurantoin 47.2% (68), trimethoprim/sulfamethoxazole 45.8% (66), cephalothin 43% (62) and amoxicillin/clavulanic acid 18% (26). Low resistance was reported to fosfomycin, amikacin and tigecycline 1.4% (2), 0.7% (1), and 0.7% (1), respectively.

Conclusions: We reported high MDR E. coli in rectal swabs of sheep in Qatar. Such resistant bacteria can potentially be transmitted to humans, resulting in public health concerns. This requires a quick response to develop and implement a stewardship program for the monitoring of antibiotic use in the veterinary in Qatar.

Published
2020-02-29
How to Cite
1.
Eltai N, Al Thani AA, Al-Hadidi SH, Abdfarag EA, Al-Romaihi H, Mahmoud MH, Alawad OK, Yassine HM (2020) Antibiotic resistance profile of commensal Escherichia coli isolated from healthy sheep in Qatar. J Infect Dev Ctries 14:138-145. doi: 10.3855/jidc.11827
Section
Original Articles