Effect of Phage-Antibiotic Synergism (PAS) in increasing antibiotic inhibition of bacteria caused of foodborne diseases

Mochammad Iqbal; Erlia Narulita; Fiqih  Zahra; Siti  Murdiyah

doi:10.3855/jidc.12094

Effect of Phage-Antibiotic Synergism (PAS) in increasing antibiotic inhibition of bacteria caused of foodborne diseases

Authors

Mochammad Iqbal Study Program of Biology Education, Faculty of Teacher Training and Learning Education, University of Jember, Jember, Indonesia
Erlia Narulita Study Program of Biology Education, Faculty of Teacher Training and Learning Education, University of Jember, Jember, Indonesia
Fiqih Zahra Study Program of Biology Education, Faculty of Teacher Training and Learning Education, University of Jember, Jember, Indonesia
Siti Murdiyah Study Program of Biology Education, Faculty of Teacher Training and Learning Education, University of Jember, Jember, Indonesia

DOI:

https://doi.org/10.3855/jidc.12094

Keywords:

Antibiotics, bacteriophages, PAS

Abstract

Introduction: Food contaminated with pathogenic bacteria is one of the most harmful things that can even threaten human life. Over time, these pathogenic bacteria are increasingly resistant to antibiotics. Continuous use of synthetic preservatives will also have an adverse effect. This study was conducted to evaluate the synergy of bacteriophage and antibiotics in increasing antibiotics inhibition to the bacteria that cause foodborne disease.

Methodology: The test was performed by plaque assay and paper disc diffusion on NA medium in the same petri dish. The combination was incubated for 24 hours at 37ºC. An antibiotic inhibition on a non-bacteriophage test showed cefadroxil could only inhibit P21B bacteria.

Results: Cefadroxil inhibition in the PAS test showed that these antibiotics could inhibit some other foodborne disease bacteria (Salmonella spp., Staphylococcus aureus, and Escherichia coli). The inhibitory observed from the clear zone located around the disc paper.

Conclusion: These results provide useful information to reduce the risk of antibiotic resistance in humans and foods.

Downloads

Published

2020-05-31

How to Cite

Iqbal M, Narulita E, Zahra F, Murdiyah S (2020) Effect of Phage-Antibiotic Synergism (PAS) in increasing antibiotic inhibition of bacteria caused of foodborne diseases. J Infect Dev Ctries 14:488–493. doi: 10.3855/jidc.12094

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 14 No. 05: May 2020

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).