Phenotypic analysis and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus in Kiambu County, Kenya

  • Sani Iliya Department of Biological Sciences, School of Pure and Applied Sciences, Mount Kenya University, Thika, Kenya https://orcid.org/0000-0001-7685-3147
  • Jonathan Mwangi School of Pharmacy and Health Sciences, United States International University-Africa, Nairobi, Kenya
  • Ronald Maathai Department of Biochemistry, Mount Kenya University, Thika, Kenya
  • Mary Muriuki School of Pure and Applied Sciences, Mount Kenya University, Thika, Kenya https://orcid.org/0000-0003-0699-3982
Keywords: Staphylococcus aureus, phenotypic characterization, antibiotic susceptibility, methicillin resistance

Abstract

Introduction: Methicillin resistant Staphylococcus aureus (MRSA) causes illness to people and can be picked up from both healthcare facilities and the environment leading to high morbidity and mortality. The study was aimed at identifying phenotypic characteristics of Methicillin-Resistant Staphylococcus aureus and determine the antibiotic susceptibility pattern of clinical samples isolated from patients attending or admitted in two health facilities in Kiambu County, Kenya.

Methodology: One hundred and thirty-eight (138) clinical samples were collected from patients attending Thika and Kiambu Level-5 Hospitals. The isolates were obtained using standard bacteriological techniques. Methicillin resistance of Staphylococcus aureus was determined using the cefoxitin disk diffusion test.

Results: Out of 138 samples, 54 (39.1%) were found to have Staphylococcus aureus of which 22 (40.7%) were shown to be MRSA using the cefoxitin- based susceptibility test. Antibiotic susceptibility testing using Kirby-Bauer technique was performed on all 54 isolates. The highest sensitivity was found in chloramphenicol 46 (85.2%) and lowest in penicillin-G 8 (14.8%). Multi-Drug Resistance (MDR) was reported in 35 (64.8%) of the 54 isolates of Staphylococcus aureus. All 22 MRSA strains were found to be MDR.

Conclusions: the data obtained revealed that there is presence of MRSA in healthcare settings in Kiambu County, Kenya with varying antibiotic sensitivity patterns as well as multidrug resistance. The findings will help healthcare workers in the county to develop preventive strategy as well as institute policy for antibiotic usage, infection control and surveillance.

Author Biography

Sani Iliya, Department of Biological Sciences, School of Pure and Applied Sciences, Mount Kenya University, Thika, Kenya

 

 

Published
2020-06-30
How to Cite
1.
Iliya S, Mwangi J, Maathai R, Muriuki M (2020) Phenotypic analysis and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus in Kiambu County, Kenya. J Infect Dev Ctries 14:597-605. doi: 10.3855/jidc.12174
Section
Original Articles