Expression of genes encoding resistance in Staphylococcus spp. isolated from bovine subclinical mastitis in Brazil

  • Eveline Zuniga Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil
  • Nilson Roberti Benites Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil https://orcid.org/0000-0001-7883-015X
  • Aline Santana da Hora Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Uberlandia (UFU), Minas Gerais, Brazil https://orcid.org/0000-0003-2999-323X
  • Priscila Luiza Mello University of Guarulhos, Guarulhos, SP, Brazil https://orcid.org/0000-0001-9089-0665
  • Marco Antonio Laes Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil https://orcid.org/0000-0002-8100-8500
  • Lilian Abgail Ribeiro de Oliveira Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil
  • Paulo Eduardo Brandão Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil https://orcid.org/0000-0003-0213-7839
  • Sheila Oliveira de Souza Silva Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil
  • Sueli Akemi Taniwaki Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil https://orcid.org/0000-0001-6355-7015
  • Priscilla Anne Melville Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil https://orcid.org/0000-0001-7819-7841
Keywords: Bovine clinical mastitis, Staphylococcus spp., Antimicrobial Resistance, Gene expression

Abstract

Introduction: Staphylococci are the most important agents associated with bovine mastitis. This study aimed at characterizing resistance factors to antimicrobials in Staphylococcus spp. isolated from the milk of cows with subclinical mastitis.

Methodology: In vitro resistance of 243 Staphylococcus spp. isolates to antimicrobials commonly used in clinical practice was evaluated. The detection and expression of genes encoding resistance mecA (gene encoding penicillin binding protein 2a) mecALGA251 (mecA homologue), blaZ (gene encoding penicillin resistance), femA and femB (genes encoding essential factors - A and B - for the expression of methicillin resistance) and aacA-aphD (gene encoding for a bifunctional enzyme that confers resistance to gentamicin) using PCR and RT-PCR was investigated.

Results: One or more genes encoding resistance to different antimicrobials were detected in 184 Staphylococcus spp. samples. The femA and femB genes were the most frequent. Regarding the variables’ detection (N = number of strains) and expression (% of strains), the following results were obtained: blaZ (N = 40 – 82.5%), femA (N = 147 – 47.6%), aacAaphD (N = 30 – 43.3%), femB (N = 138 – 29.7%), mecA (N = 33 – 27.3%), mecALGA251 (N = 01 – 0.0%). There was a higher occurrence of phenotypic resistant strains for amoxicillin, ampicillin and penicillin in isolates positive for detection and/or expression of blaZ gene when compared with the other genes.

Conclusions: The present study provides new information on genotypic traits of Staphylococcus isolates from bovine subclinical mastitis especially regarding the evaluation of expression of genes associated with antimicrobial resistance in Staphylococcus spp. using molecular tools.

Published
2020-07-31
How to Cite
1.
Zuniga E, Roberti Benites N, da Hora AS, Mello PL, Laes MA, de Oliveira LAR, Brandão PE, Silva SO de S, Taniwaki SA, Melville PA (2020) Expression of genes encoding resistance in Staphylococcus spp. isolated from bovine subclinical mastitis in Brazil. J Infect Dev Ctries 14:772-780. doi: 10.3855/jidc.12611
Section
Original Articles