Flea-borne Rickettsia species in fleas, Caldas department, Colombia

  • Carol B Colonia Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia
  • Alejandro Ramírez-Hernández Grupo Parasitología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá D.C., Colombia
  • Juliana Gil-Mora Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia
  • Juan C Agudelo Facultad de Ciencias Agropecuarias, Universidad de Caldas, Manizales, Caldas, Colombia
  • Gabriel Jaime Castaño Villa Grupo de investigación GEBIOME, Departamento de Desarrollo Rural y Recursos Naturales, Facultad de Ciencias Agropecuarias, Universidad de Caldas, Manizales, Caldas, Colombia
  • Camilo Pino Laboratorio de Investigación en Sistemas Inteligentes, Facultad de Ingeniería, Universidad Nacional de Colombia, Bogotá D.C., Colombia
  • Paola Betancourt-Ruiz Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia
  • Jorge E Pérez Cárdenas Facultad de Ciencias para la Salud, Universidad de Caldas, Manizales, Caldas, Colombia
  • Lucas S Blanton Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, United States
  • Marylin Hidalgo Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia
Keywords: Rickettsia felis, Rickettsia asembonensis, RFLP, vector-borne diseases, zoonotic diseases

Abstract

Introduction: Rickettsioses are zoonotic diseases caused by pathogenic bacteria of the genus Rickettsia and transmitted to man by means of arthropod vectors such as ticks, fleas, mites and lice. Historically, Caldas Department has reported a significant number of cases of murine typhus to the Colombian national health surveillance system, and consequent studies of flea-borne rickettsiosis identified the circulation of Rickettsia typhi and Rickettsia felis in multiple municipalities. Our aim was to genotype species of Rickettsia detected in fleas collected from domestic and wild mammals in Caldas.

Methodology: Flea samples were taken by convenience sampling from dogs, cats and wild mammals (rodents and marsupials) in 26 municipalities. Specimens were classified by current taxonomic keys and pooled for DNA extraction and molecular screening for Rickettsia spp. by PCR amplification of gltA, htrA and sca5 genes. Positive samples were genotyped by enzyme digestion (htrA) and sequencing.

Results: A total of 1388 flea samples were collected. Rickettsia DNA was amplified in 818 (gltA), 883 (htrA) and 424 (sca5) flea pools. Alignment analysis with available Rickettsia DNA sequences showed greater similarity with R. asembonensis (gltA) and with R. felis (sca5 and htrA). Restriction pattern was compatible with R. felis. R. typhi was not identified.

Conclusion: The present study confirms the presence and high prevalence of R. asembonensis and R. felis in fleas from domestic and wild animals in different municipalities from Caldas Department.

Published
2020-10-31
How to Cite
1.
Colonia CB, Ramírez-Hernández A, Gil-Mora J, Agudelo JC, Castaño Villa GJ, Pino C, Betancourt-Ruiz P, Pérez Cárdenas JE, Blanton LS, Hidalgo M (2020) Flea-borne Rickettsia species in fleas, Caldas department, Colombia. J Infect Dev Ctries 14:1155-1163. doi: 10.3855/jidc.12524
Section
Original Articles