Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna

  • Dramane Kiemde Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
  • Inês Ribeiro Heidelberg Institute of Global Health (HIGH), Heidelberg University Hospital, Germany
  • Soufiane Sanou Department of Medical Bacteriology and Virology, National Reference Laboratory for Antimicrobial Resistance, University Hospital Centre Sanou Sourou, Bobo Dioulasso, Burkina Faso
  • Boubacar Coulibaly Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
  • Ali Sie Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
  • Abdoul-Salam Ouedraogo Department of Medical Bacteriology and Virology, National Reference Laboratory for Antimicrobial Resistance, University Hospital Centre Sanou Sourou, Bobo Dioulasso, Burkina Faso
  • Aurélia Souares German Center for Infection Research (DZIF), Heidelberg, Hamburg-Lübeck-Borstel-Riems, Heidelberg, Germany
  • Daniel Eibach Bernhard Nocht Institute for Tropical Medicine (BNITM), Hamburg, Germany
Keywords: Enterobacteriaceae, Escherichia coli, extended-spectrum beta-lactamase, ESBL

Abstract

Introduction: Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resistance in Enterobacteriaceae. The purpose of this study is to characterize the ESBL genes produced by community-acquired uropathogenic Escherichia coli strains in the Nouna District, in the West-African country, Burkina Faso.

Methodology: Samples were collected from non-hospitalized patients who came for consultation at the CMA (Centre Médical avec Antenne chirurgicale) in Nouna and were sent to the laboratory for a urine culture test. The detection of ESBL production by the bacteria was carried out with the double-disc synergy test and the extraction of the ESBL genes with the heat shock method. Molecular characterization of ESBL genes was performed with three sequential multiplex polymerase chain reaction (PCR) assays.

Results: One hundred and eighty-two (182) bacteriological cultures were analyzed and 29 E. coli isolated, between 01/07/2017 and 01/07/2018. The ESBL phenotype was found in 13/29 (44.8%). Multiplex PCR yielded many beta-lactamase genes, predominantly blaCTX-M-1,3,15 (12/13; 92.3%) followed by beta-lactamase genes blaOXA-1,4,30 (8/13; 61.5%) and beta-lactamase genes blaTEM-1,2 (7/13; 53.8%).

Conclusion: This study showed that the blaCTX-M-1,3,15 genes produced by uropathogenic E. coli were predominant. Sequencing of these genes would be needed to better characterize the different types of ESBL circulating in Nouna.

Published
2020-11-30
How to Cite
1.
Kiemde D, Ribeiro I, Sanou S, Coulibaly B, Sie A, Ouedraogo A-S, Souares A, Eibach D (2020) Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna. J Infect Dev Ctries 14:1274-1280. doi: 10.3855/jidc.11737
Section
Original Articles