Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna

Dramane Kiemde; Inês Ribeiro; Soufiane Sanou; Boubacar Coulibaly; Ali Sie; Abdoul-Salam Ouedraogo; Aurélia Souares; Daniel Eibach

doi:10.3855/jidc.11737

Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna

Authors

Dramane Kiemde Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
Inês Ribeiro Heidelberg Institute of Global Health (HIGH), Heidelberg University Hospital, Germany
Soufiane Sanou Department of Medical Bacteriology and Virology, National Reference Laboratory for Antimicrobial Resistance, University Hospital Centre Sanou Sourou, Bobo Dioulasso, Burkina Faso
Boubacar Coulibaly Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
Ali Sie Nouna Health Research Center/CMA (Centre Médical avec Antenne Chirurgicale) Biological Analysis Laboratory, Burkina Faso
Abdoul-Salam Ouedraogo Department of Medical Bacteriology and Virology, National Reference Laboratory for Antimicrobial Resistance, University Hospital Centre Sanou Sourou, Bobo Dioulasso, Burkina Faso
Aurélia Souares German Center for Infection Research (DZIF), Heidelberg, Hamburg-Lübeck-Borstel-Riems, Heidelberg, Germany
Daniel Eibach Bernhard Nocht Institute for Tropical Medicine (BNITM), Hamburg, Germany

DOI:

https://doi.org/10.3855/jidc.11737

Keywords:

Enterobacteriaceae, Escherichia coli, extended-spectrum beta-lactamase, ESBL

Abstract

Introduction: Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resistance in Enterobacteriaceae. The purpose of this study is to characterize the ESBL genes produced by community-acquired uropathogenic Escherichia coli strains in the Nouna District, in the West-African country, Burkina Faso.

Methodology: Samples were collected from non-hospitalized patients who came for consultation at the CMA (Centre Médical avec Antenne chirurgicale) in Nouna and were sent to the laboratory for a urine culture test. The detection of ESBL production by the bacteria was carried out with the double-disc synergy test and the extraction of the ESBL genes with the heat shock method. Molecular characterization of ESBL genes was performed with three sequential multiplex polymerase chain reaction (PCR) assays.

Results: One hundred and eighty-two (182) bacteriological cultures were analyzed and 29 E. coli isolated, between 01/07/2017 and 01/07/2018. The ESBL phenotype was found in 13/29 (44.8%). Multiplex PCR yielded many beta-lactamase genes, predominantly bla_CTX-M-1,3,15 (12/13; 92.3%) followed by beta-lactamase genes bla_OXA-1,4,30 (8/13; 61.5%) and beta-lactamase genes bla_TEM-1,2 (7/13; 53.8%).

Conclusion: This study showed that the bla_CTX-M-1,3,15 genes produced by uropathogenic E. coli were predominant. Sequencing of these genes would be needed to better characterize the different types of ESBL circulating in Nouna.

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Published

2020-11-30

How to Cite

Kiemde D, Ribeiro I, Sanou S, Coulibaly B, Sie A, Ouedraogo A-S, Souares A, Eibach D (2020) Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna. J Infect Dev Ctries 14:1274–1280. doi: 10.3855/jidc.11737

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Issue

Vol. 14 No. 11: November 2020

Section

Original Articles

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