Phenotypic and genotypic characterization of multidrug-resistant Acinetobacter baumannii isolated in Algerian hospitals

  • Nabila Benamrouche Medical Bacteriology Laboratory, Institut Pasteur of Algeria, Algiers, Algeria
  • Ourida Lafer Medical Bacteriology Laboratory, Institut Pasteur of Algeria, Algiers, Algeria
  • Lahcen Benmahdi University Regional Military Hospital of Oran, Oran, Algeria
  • Akila Benslimani Central Laboratory, Specialized Hospital Establishment Maouche Mohand Amokrane, Algiers, Algeria
  • Wahiba Amhis Central Laboratory, Public Hospital Establishment Ibn Ziri of Bologhine, Algiers, Algeria
  • Houria Ammari Central Laboratory, University Hospital of Beni Messous, Algiers, Algeria
  • Farida Assaous Medical Bacteriology Laboratory, Institut Pasteur of Algeria, Algiers, Algeria
  • Amina Azzam Microbiology and Parasitology Laboratory, University Hospital of Tizi Ouzou, Tizi Ouzou, Algeria
  • Kheira Rahal Medical Bacteriology Laboratory, Institut Pasteur of Algeria, Algiers, Algeria
  • Hassiba Tali Maamar Medical Bacteriology Laboratory, Institut Pasteur of Algeria, Algiers, Algeria
Keywords: Multidrug-resistant Acinetobacter baumannii, blaCTX-M-15, blaSHV-33, blaNDM-1, colistin-resistance, Algeria

Abstract

Introduction: The aim of this study was to investigate the drug-resistance and the molecular characterization of carbapenemases, ESBL, and aminoglycoside-modifying enzymes among Acinetobacter baumannii clinical isolates in Algerian hospitals.

Methodology: A total of 92 A. baumannii isolates were collected between 2012 and 2016. Antimicrobial susceptibility testings were performed for β-lactams, aminoglycosides, fluoroquinolones, trimethoprim-sulfamethoxazole, rifampicin and colistin. The phenotypic characterization of β-lactamases was investigated. For 30 randomly targeted strains, the carriage of the carbapenemases, ESBL and aminoglycoside-modifying enzymes -encoding genes was determined by PCR. Sequencing was carried out for carbapenemases and ESBL genes.

Results: Most of the 92 isolates studied were recovered from hospitalized patients (93.5%) and were mainly from intensive care units (51.1%) and orthopedics (19.6%). The strains were collected primarily from low respiratory tract (33.7%), wounds (23.9%) and urine (16.3%). Multidrug-resistant A. baumannii strains were prevalent (96.7%). High rates of resistance were observed for almost all antibiotics tested (>70%) excluding rifampicin (7.6%) and colistin (5.4%). For the five colistin-resistant strains, MICs ranged between 4 and 128 µg/mL. Positive MBL (83.7%) and ESBL (23.9%) strains were identified. Regarding β-lactams, the blaNDM and both blaSHV and blaCTX-M1 genes were detected in five and two strains respectively. Sequencing of the genes revealed the presence of blaNDM-1, blaCTX-M-15, and blaSHV-33. For aminoglycosides, aac(6’)-Ib, ant(2’’)-I and aph(3’)-VI genes were detected in three, seven and six strains respectively.

Conclusions: Here, we report the first co-occurrence of extended-spectrum β-lactamases SHV-33 and CTX-M-15, the carbapenemase NDM-1 and the emergence of colistin-resistant A. baumannii in Algerian hospitals.

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Published
2020-12-31
How to Cite
1.
Benamrouche N, Lafer O, Benmahdi L, Benslimani A, Amhis W, Ammari H, Assaous F, Azzam A, Rahal K, Tali MaamarH (2020) Phenotypic and genotypic characterization of multidrug-resistant Acinetobacter baumannii isolated in Algerian hospitals. J Infect Dev Ctries 14:1395-1401. doi: 10.3855/jidc.12348
Section
Original Articles