Quantification of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp. in bacterial vaginosis

  • Nedzib Numanovic Department of Gynecology, General Hospital Novi Pazar, Novi Pazar, Serbia
  • Snezana Ribis Department of Microbiology, University Clinical Center Kragujevac, Kragujevac, Serbia
  • Jelena Cukic Department of Microbiology, Public Health Institute, Kragujevac, Serbia
  • Dane Nenadic Department of Gynecology, Military Medical Academy, Belgrade, Serbia
  • Aleksandar Zivanovic Department of Gynecology, Faculty of Medical Sciences, Kragujevac, Serbia
  • Predrag Sazdanovic Department of Gynecology, Faculty of Medical Sciences, Kragujevac, Serbia
  • Violeta Ninkovic Department of Microbiology, Public Health Institute, Kragujevac, Serbia
  • Dejan Baskic Department of Microbiology, Public Health Institute, Kragujevac, Serbia
Keywords: bacterial vaginosis, Amsel, real-time PCR, G. vaginalis, A. vaginae

Abstract

Introduction: The aim of the study was to investigate prevalence of bacteria most frequently associated with bacterial vaginosis using Amsel’s criteria as well as to quantify these bacteria by real-time PCR and to explore the difference in their quantity between healthy and bacterial vaginosis samples.

Methodology: For classification of vaginal discharge samples Amsel’s criteria have been used. To detect and quantify Gardnerella vaginalis Atopobium vaginae, Lactobacillus spp. and total vaginal microbiome, real-time PCR has been applied.

Results: According to results of our study Amsel’s criteria matched well with real-time PCR diversification of healthy women and women with BV. Nevertheless, real-time PCR has been more sensitive in diagnosis of bacterial vaginosis. DNA quantification of bacteria demonstrated that mutual abundance of G.vaginalis and A. vaginae was good bacterial vaginosis marker . On the contrary, Lactobacillus spp. was present in high amount in both healthy and bacterial vaginosis samples, but ratio of investigated bacteria was different between them. In fact, G. vaginalis and A. vaginae comprised only 0.1% of total microbiome in healthy, whereas Lactobacillus spp. took 99.3% of it. Nonetheless, in bacterial vaginosis, G. vaginalis and A. vaginae made up 34.4% of total microbiome, while Lactobacillus spp. was 21.6%.

Conclusions: According to the results of our study real-time PCR analysis was more sensitive in diagnosis of bacterial vaginosis than Amsel’s method, as well as it represented fine tool in making a difference between microbial entities in healthy and bacterial vaginosis samples.

Published
2021-09-30
How to Cite
1.
NumanovicN, Ribis S, Cukic J, Nenadic D, ZivanovicA, Sazdanovic P, NinkovicV, Baskic D (2021) Quantification of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp. in bacterial vaginosis. J Infect Dev Ctries 15:1293-1298. doi: 10.3855/jidc.13091
Section
Original Articles