Characterization of commensal Escherichia coli isolates from slaughtered sheep in Mexico

  • Jorge Acosta-Dibarrat Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México https://orcid.org/0000-0003-2901-4740
  • Edgar Enriquez-Gómez Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México
  • Martín Talavera-Rojas Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México http://orcid.org/0000-0003-0908-985X
  • Edgardo Soriano-Vargas Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México http://orcid.org/0000-0002-1514-1741
  • Armando Navarro Department of Public Health, Faculty of Medicine, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Colonia Copilco, Delegación Coyoacan, México City, México http://orcid.org/0000-0002-6693-7168
  • Rosario Morales-Espinosa Laboratory of Bacterial Genomics, Department of Microbiology and Parasitology, Faculty of Medicine, Universidad Nacional Autónoma de México, Colonia Copilco, México City, México http://orcid.org/0000-0003-2110-4397
Keywords: commensal E. coli, slaughtered, sheep, México

Abstract

Introduction: Commensal Escherichia coli is defined as bacteria without known virulence factors that could be playing a specific role in some diseases; however, they could be responsible to disseminate antimicrobial resistance genes to other microorganisms. This study aimed to characterize the commensal E. coli isolates obtained from slaughtered sheep in the central region of Mexico.

Methodology: Isolates were classified as commensal E. coli when distinctive genes related to diarrheagenic pathotypes (stx1, stx2, eae, bfp, LT, stp, ipaH, and aggR) were discarded by PCR. Identification of serotype, phylogenetic group, and antimicrobial resistance was also performed.

Results: A total of 41 isolates were characterized. The phylogenetic groups found were B1 in 37 isolates (90.2%), A in 2 (4.8%), and 1 isolate (2.4%) for C and D groups. Serotypes associated with diarrhea in humans (O104:H2 and O154:NM) and hemolytic uremic syndrome (O8:NM) were detected. Thirty-three isolates (80%) were resistant to ceftazidime, 23 (56%), to tetracycline 8 (19.5%) to ampicillin, and 1 to amikacin. Six isolates (14.6%) were multidrug-resistant.

Conclusions: This study provides new information about commensal E. coli in slaughtered sheep, high percentages of resistance to antibiotics, and different profiles of antimicrobial resistance were found, their dissemination constitute a risk factor towards the consuming population.

Author Biographies

Edgar Enriquez-Gómez, Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México

PhD student

Martín Talavera-Rojas, Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México

research professor

Edgardo Soriano-Vargas, Center for Research and Advanced Studies in Animal Health. Faculty of Veterinary Medicine and Zootechnics. Universidad Autónoma del Estado de México. Toluca, Estado de México, México

research professor

Armando Navarro, Department of Public Health, Faculty of Medicine, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Colonia Copilco, Delegación Coyoacan, México City, México

Laboratory Head

Rosario Morales-Espinosa , Laboratory of Bacterial Genomics, Department of Microbiology and Parasitology, Faculty of Medicine, Universidad Nacional Autónoma de México, Colonia Copilco, México City, México

Laboratory Head

Published
2021-11-30
How to Cite
1.
Acosta-DibarratJ, Enriquez-GómezE, Talavera-RojasM, Soriano-VargasE, Navarro A, Morales-Espinosa R (2021) Characterization of commensal Escherichia coli isolates from slaughtered sheep in Mexico. J Infect Dev Ctries 15:1755-1760. doi: 10.3855/jidc.14001
Section
Brief Original Articles