Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia

Ivana Buha; Vesna Škodrić-Trifunović; Marina Anđelković; Sonja Pavlović; Miša Vreća; Mihailo Stjepanović; Jelena Milin-Lazović; Marko Simić; Gordana Antonijević; Vesna Spasovski

doi:10.3855/jidc.16721

Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia

Authors

Ivana Buha Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia https://orcid.org/0000-0002-5400-6016
Vesna Škodrić-Trifunović Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia
Marina Anđelković Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Sonja Pavlović Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Miša Vreća Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Mihailo Stjepanović Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia https://orcid.org/0000-0003-1787-1438
Jelena Milin-Lazović Department for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
Marko Simić Clinic for Orthopedic Surgery and Traumatology, Clinical Center of Serbia, Belgrade, Serbia
Gordana Antonijević Special Hospital for Lung Diseases Ozren, Soko banja, Serbia
Vesna Spasovski Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

DOI:

https://doi.org/10.3855/jidc.16721

Keywords:

miRNA-146a gene expression, biomarker, active pulmonary tuberculosis

Abstract

Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity.

Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB.

Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups.

Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB.

Downloads

Published

2022-08-30

How to Cite

Buha I, Škodrić-Trifunović V, Anđelković M, Pavlović S, Vreća M, Stjepanović M, Milin-Lazović J, Simić M, Antonijević G, Spasovski V (2022) Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia. J Infect Dev Ctries 16:1317–1322. doi: 10.3855/jidc.16721

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 16 No. 08: August 2022

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).