Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia

  • Ivana Buha Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia https://orcid.org/0000-0002-5400-6016
  • Vesna Škodrić-Trifunović Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia
  • Marina Anđelković Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Sonja Pavlović Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Miša Vreća Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
  • Mihailo Stjepanović Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia https://orcid.org/0000-0003-1787-1438
  • Jelena Milin-Lazović Department for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
  • Marko Simić Clinic for Orthopedic Surgery and Traumatology, Clinical Center of Serbia, Belgrade, Serbia
  • Gordana Antonijević Special Hospital for Lung Diseases Ozren, Soko banja, Serbia
  • Vesna Spasovski Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Keywords: miRNA-146a gene expression, biomarker, active pulmonary tuberculosis

Abstract

Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity.

Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB.

Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups.

Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB.

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Published
2022-08-30
How to Cite
1.
Buha I, Škodrić-TrifunovićV, Anđelković M, Pavlović S, Vreća M, Stjepanović M, Milin-LazovićJ, Simić M, Antonijević G, Spasovski V (2022) Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia. J Infect Dev Ctries 16:1317-1322. doi: 10.3855/jidc.16721
Section
Original Articles

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