Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China

Xian Zhang; Jian Xue; Mei-Jing Shen; Wen-Hong An; Ze-Qiong Chen; Kai-Feng Wu

doi:10.3855/jidc.17003

Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China

Authors

Xian Zhang Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
Jian Xue Department of Health Management, Zunyi Medical And Pharmaceutical College, Guizhou, China
Mei-Jing Shen Department of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
Wen-Hong An Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
Ze-Qiong Chen Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
Kai-Feng Wu Department of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China

DOI:

https://doi.org/10.3855/jidc.17003

Keywords:

Whole genome sequencing, paediatrics, carbapenem-resistant Klebsiella pneumoniae, molecular typing, drug resistance

Abstract

Introduction: There are few studies on paediatric carbapenem-resistant Klebsiella pneumoniae (CRKP) in China. The present study investigated the molecular epidemiological and drug resistance characteristics of CRKP from paediatric patients in China to provide a reference for the prevention and control of CRKP infection.

Methodology: In total, 20 nonrepetitive clinical CRKP isolates were collected between February 2019 and February 2020 in a tertiary hospital in China. Strain identification and drug susceptibility testing were carried out using the VITEK^® 2 Compact Bacterial Identification and Monitoring System. Sequence typing, phylogenetic relationships, and antibiotic resistance-associated genes were analysed by whole genome sequencing (WGS).

Results: sequence typing (MLST) and Core genome multilocus sequence typing (cgMLST) analysis revealed the most frequently represented were ST2407-CT3536 (30%), ST76-CT5893 (25%), and ST309-CT7864 (25%). All 20 CRKP isolates were divided into three clusters. All isolates were highly resistant to a variety of β-lactams and were highly susceptible to quinolones, aminoglycosides, and sulphonamides. All isolates mainly carried the carbapenem resistance genes bla_NDM-1 and bla_KPC-2, among which 10 isolates carried both bla_NDM-1 and bla_KPC-2 simultaneously.

Conclusions: Sequence typing, phylogenetic relationships, and antibiotic resistance genes can be determined using WGS technology. This can guide CRKP infection control and clinical treatment for paediatric patients.

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Published

2022-11-29

How to Cite

Zhang X, Xue J, Shen M-J, An W-H, Chen Z-Q, Wu K-F (2022) Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China. J Infect Dev Ctries 16:1726–1731. doi: 10.3855/jidc.17003

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Issue

Vol. 16 No. 11: November 2022

Section

Original Articles

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