Prevalence and antimicrobial susceptibility profile of Salmonella isolated from food products in the region of Casablanca, Morocco

Authors

  • Chaima Sabri Laboratory of Natural Resources and Sustainable Development, Research Unit on Metabolism, Physiology, and Nutrition, Department of Biology, Faculty of Science, Ibn Tofail University, Kenitra, Morocco
  • Bouchra Karraouan Laboratory of food microbiology, Institut Pasteur du Maroc, Casablanca, Morocco
  • Thami Berradi Regional Analysis Laboratory and Research, National Health Security Office Foodstuffs, Casablanca, Morocco https://orcid.org/0000-0002-6812-1859
  • Brahim Bouchrif Laboratory of food microbiology, Institut Pasteur du Maroc, Casablanca, Morocco
  • Fatiha El Mellouli Regional Analysis Laboratory and Research, National Health Security Office Foodstuffs, Casablanca, Morocco
  • Nadia Ziyate Division of Pharmacy and Veterinary Inputs, Control and Expertises Service, National Health Security Office foodstuffs, Rabat, Morocco
  • Sara Razzak Laboratory of Natural Resources and Sustainable Development, Research Unit on Metabolism, Physiology, and Nutrition, Department of Biology, Faculty of Science, Ibn Tofail University, Kenitra, Morocco https://orcid.org/0000-0002-5141-6052
  • Bouchra Taib Laboratory of Natural Resources and Sustainable Development, Research Unit on Metabolism, Physiology, and Nutrition, Department of Biology, Faculty of Science, Ibn Tofail University, Kenitra, Morocco
  • Youness Taboz Laboratory of Natural Resources and Sustainable Development, Research Unit on Metabolism, Physiology, and Nutrition, Department of Biology, Faculty of Science, Ibn Tofail University, Kenitra, Morocco https://orcid.org/0000-0003-2485-9592

DOI:

https://doi.org/10.3855/jidc.17236

Keywords:

Salmonella, serovars, prevalence, antimicrobial resistance, Morocco

Abstract

Introduction: Salmonellosis is a foodborne bacterial disease responsible for food epidemics around the world. The objective of this study is to determine the prevalence and diversity of Salmonella serotypes in several food products isolated at the Casablanca Regional Analysis and Research Laboratory and to test their resistance to different antimicrobials.

Methodology: The isolation and identification of Salmonella were performed according to Moroccan standard 08.0.116. All isolates were serotyped and were then tested for antibiotic resistance using the disk diffusion method. The Salmonella isolates were further analyzed by PCR to detect the presence of virulence genes invA.

Results:20 different serotypes were identified from 80 strains isolated from 2015 to 2019, the most common of which are S. kentucky (26.3%) followed by S. muenster (10%), S. typhimurium (8.7%), S. menston (7.5%) and S. enteritidis (6.3%). Antimicrobial susceptibility testing revealed that 66.25% of isolates were resistant to at least one of the 14 antimicrobial agents tested. Bacterial resistance was most frequently observed for tetracycline with 46.25%, 45% to sulfonamide, 35% to nalidixic acid, 26, 25% to ampicillin, and 25% to ciprofloxacin. Salmonella serotypes S. montevideo, S. virchow, S. amsterdam, S. anatum, and S. bloomsbury were 100% susceptible to all antimicrobials tested. Examination of Salmonella for invA gene was positive for all the strains.

Conclusions: The results of this study have shown that minced meat has a high level of Salmonella contamination, which can be considered one of the main potential sources of human salmonellosis in Morocco.

Author Biography

Brahim Bouchrif, Laboratory of food microbiology, Institut Pasteur du Maroc, Casablanca, Morocco

Departement sécurite alimentaire et environnement

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Published

2023-03-31

How to Cite

1.
Sabri C, Karraouan B, Berradi T, Bouchrif B, El Mellouli F, Ziyate N, Razzak S, Taib B, Taboz Y (2023) Prevalence and antimicrobial susceptibility profile of Salmonella isolated from food products in the region of Casablanca, Morocco. J Infect Dev Ctries 17:388–396. doi: 10.3855/jidc.17236

Issue

Vol. 17 No. 03: March 2023

Section

Original Articles

License

Copyright (c) 2023 CHAIMA SABRI, BOUCHRA KARRAOUAN , Thami Berradi, BRAHIM BOUCHRIF, FATIHA EL MELLOULI, NADIA ZIYATE, SARA RAZZAK, BOUCHRA TAIB , YOUNESS TABOZ

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