TB specific intracellular cytokines production in Synovial liquid for diagnosis of tuberculous arthritis

Authors

  • Serena Vita Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy
  • Claudia Mascia Sapienza University of Rome, Rome, Italy
  • Blerta Kertusha Sapienza University of Rome, Rome, Italy
  • Raffaella Marocco Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy
  • Ilaria Sauzullo Sapienza University of Rome, Rome, Italy
  • Fabio Mengoni Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy
  • Irene Pozzetto Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy
  • Alessandro Scalzi Orthopedics and Traumatology, Santa Maria Goretti Hospital, Latina, Italy
  • Parni Nijhawan Sapienza University of Rome, Rome, Italy
  • Stefano Savinelli Centre for Experimental Pathogen Host Research, University College Dublin, Dublin, Ireland
  • Laura Fondaco Sapienza University of Rome, Rome, Italy
  • Anna Carraro Sapienza University of Rome, Rome, Italy
  • Cosmo Del Borgo Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy
  • Claudio Maria Mastroianni Sapienza University of Rome, Rome, Italy
  • Miriam Lichtner Infectious Disease Unit, Santa Maria Goretti Hospital, Latina, Italy

DOI:

https://doi.org/10.3855/jidc.12618

Keywords:

Tuberculous arthritis, TB diagnosis, intracellular cytokines

Abstract

Introduction: Skeletal tuberculosis (TB) accounts for about 10 to 35% of extrapulmonary cases and the knee is the most frequent site after the spine and hip. The diagnosis is difficult and largely clinical.

Case presentation: This is a case of a young Pakistani man with a history of joint pain for about 4 years, who was diagnosed with chronic arthritis of the right knee. Microscopy of synovial fluid and conventional diagnostic tests to identify Mycobacterium tuberculosis were negative, while a non-classical method based on intracellular cytokine flow cytometry response of CD4 T-cells in synovial fluid helped us to address the diagnosis, which was subsequently confirmed by Polymerase Chain Reaction (PCR).

Conclusions: Thanks to an innovative immunological approach, supported by PCR for detection of M. tuberculosis DNA, we were able to diagnose tuberculous arthritis of the knee, which allowed prompt initiation of treatment to reduce morbidity and mortality.

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Published

2023-12-31

How to Cite

1.
Vita S, Mascia C, Kertusha B, Marocco R, Sauzullo I, Mengoni F, Pozzetto I, Scalzi A, Nijhawan P, Savinelli S, Fondaco L, Carraro A, Del Borgo C, Mastroianni CM, Lichtner M (2023) TB specific intracellular cytokines production in Synovial liquid for diagnosis of tuberculous arthritis. J Infect Dev Ctries 17:1829–1833. doi: 10.3855/jidc.12618

Issue

Vol. 17 No. 12: December 2023

Section

Case Reports

License

Copyright (c) 2023 Serena Vita, Claudia MAscia, Blerta Kertusha, Raffaella Marocco, ilaria sauzullo, Fabio Mengoni, Irene pozzetto, Alessandro Sclazi, Laura Fondaco, anna carraro, Cosmo Del Borgo, claudio maria mastroianni, miriam lichtner

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