Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis

Authors

  • Dong Li Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China https://orcid.org/0009-0005-3091-2478
  • Yinghao Yang Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China https://orcid.org/0009-0003-0478-536X
  • Ying Xie Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China
  • Jianrong Wang Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China
  • Wensheng Xu Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China
  • Junxue Wang Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China
  • Xiaofeng Hang Department of Infectious Diseases, Changzheng Hospital, Naval Medical University, Shanghai, China https://orcid.org/0000-0001-9222-5140

DOI:

https://doi.org/10.3855/jidc.18235

Keywords:

Sepsis, bloodstream infection, next generation sequencing, High-Throughput Nucleotide Sequencing

Abstract

Introduction: Systematic evaluation of the diagnostic value of next generation sequencing (NGS) in sepsis etiology.

Methodology: We conducted a systematic search on four databases (Web of Science, Cochrane, PubMed, and Embase) and compiled diagnostic experiments using NGS to evaluate sepsis etiology. Two researchers conducted research and obtained data independently.

Results: Nine documents were included comprising 747 patients, 988 blood samples, 175 bronchoalveolar lavage fluid (BALF) samples, 16 cerebrospinal fluid samples, and one urine sample. The combined sensitivity of each study was 0.89 (95% CI: 0.82-0.95). The combined specificity was 0.40 (95% CI: 0.25-0.55). The combined positive likelihood ratio was 1.51 (95% CI: 1.18-1.98). The combined negative likelihood ratio was 0.28 (95% CI: 0.11-0.48). The diagnostic odds ratio (DOR) was 6.38 (95% CI: 2.53-15.32) and the area under the curve (AUC) was 0.84, (95% CI: 0.62-0.94).

Conclusions: Based on the data we collected, we found that compared with the blood culture technology, NGS has the advantages of high sensitivity and wide detection range, but its specificity was low. Further study is needed to confirm the value of NGS in the etiological diagnosis of patients with sepsis.

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Published

2023-12-31

How to Cite

1.
Li D, Yang Y, Xie Y, Wang J, Xu W, Wang J, Hang X (2023) Evaluation of the diagnostic effectiveness of next generation sequencing in sepsis etiology: a systematic review and meta-analysis. J Infect Dev Ctries 17:1667–1673. doi: 10.3855/jidc.18235

Issue

Vol. 17 No. 12: December 2023

Section

Reviews

License

Copyright (c) 2023 Dong Li, Yinghao Yang, Ying Xie, Jianrong Wang, Wensheng Xu, Junxue Wang, Xiaofeng Hang

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