Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan

AbdelRahman M Zueter; Taghrid Mharib; Dalal Shqair; Mohammad Al-Tamimi; Hana M Sawan; Amani Zaiter; Hadeel Albalawi; Dua’a Al Balawi

doi:10.3855/jidc.18810

Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan

Authors

AbdelRahman M Zueter Department of Medical Laboratory Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan https://orcid.org/0000-0001-9960-4290
Taghrid Mharib Department of Medical Laboratory Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan
Dalal Shqair Department of Basic Medical Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan
Mohammad Al-Tamimi Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan https://orcid.org/0000-0001-7486-4192
Hana M Sawan Faculty of Pharmacy, Zarqa University, Zarqa, Jordan
Amani Zaiter Department of Science, Omareyah School, Amman, Jordan
Hadeel Albalawi Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan
Dua’a Al Balawi Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan

DOI:

https://doi.org/10.3855/jidc.18810

Keywords:

Escherichia coli, Jordan, UTI, MLST, Sequence type

Abstract

Introduction: Escherichia coli (E. coli) is the major cause of extraintestinal infections in the urinary tracts and bloodstream in humans in the community and health care institutions. Several studies on the genetic characterization of E. coli among clinical and environmental isolates were performed and revealed a wide diversity of sequence types (STs). In Jordan, phenotypic and genetic features of E. coli were extensively studied but there is still a need to identify the STs that inhabit the community.

Methodology: In this study, multi-locus sequence typing (MLST) was performed on archived clinical E. coli isolates collected from different hospitals in Jordan and the identified STs were extensively analyzed.

Results: Genotyping of 92 E. coli isolates revealed 34 STs and 9 clonal complexes. The frequencies of STs ranged between 1 to 23 observations. The most frequent STs among E. coli isolates were ST131 (n = 23), ST69 (n = 19), ST998 (n = 7), ST2083 (n = 5), and ST540 (n = 4). These five ST accounted for up to 60% of the 92 E. coli isolates. Based on the MLST database, the STs reported in this work were world widely recognized in humans, animals, and in the environment.

Conclusions: This study has elaborated more knowledge about the genotypes of E. coli in Jordan, with recommendations for future studies to correlate its genotypes with virulence and resistance genes.

Downloads

Published

2024-04-30

How to Cite

Zueter AM, Mharib T, Shqair D, Al-Tamimi M, Sawan HM, Zaiter A, Albalawi H, Al Balawi D (2024) Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan. J Infect Dev Ctries 18:571–578. doi: 10.3855/jidc.18810

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 18 No. 04: April 2024

Section

Original Articles

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).